PMID- 9106730
OWN - NLM
STAT- MEDLINE
DCOM- 19970703
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 8
IP  - 3
DP  - 1997 Feb 10
TI  - Oleic acid enhances ACh receptor currents by activation of 
      Ca2+/calmodulin-dependent protein kinase II.
PG  - 597-601
AB  - Oleic acid, a cis-unsaturated free fatty acid, is proposed to be involved in the 
      protein kinase C (PKC) activation pathway. Its biological actions, however, have 
      not been well-characterized. We examined the effects of oleic acid on 
      acetylcholine (ACh)-gated channel currents using Torpedo nicotinic ACh receptors 
      expressed in Xenopus oocytes. Oleic acid (10 microM) enhanced the currents, 
      reaching a maximum (140%) 20 min after treatment, while no enhancement was 
      observed in Ca(2+)-free extracellular solution. The current potentiation by oleic 
      acid was not inhibited by PKC inhibitors such as PKCI or GF109203X. Furthermore, 
      oleic acid potentiated the currents in mutant ACh receptors lacking potential PKC 
      phosphorylation sites. In contrast, the potentiation was fully inhibited by a 
      CaMKII inhibitor, KN-62. These results strongly suggest that oleic acid 
      potentiates ACh receptor currents by activation of calmodulin-dependent protein 
      kinase II (CaMKII), independent of the PKC pathway.
FAU - Nishizaki, T
AU  - Nishizaki T
AD  - Department of Physiology, Kobe University School of Medicine, Japan.
FAU - Ikeuchi, Y
AU  - Ikeuchi Y
FAU - Matsuoka, T
AU  - Matsuoka T
FAU - Sumikawa, K
AU  - Sumikawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Maleimides)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (Recombinant Proteins)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 452VLY9402 (Serine)
RN  - 63HM46XPOW (KN 62)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - L79H6N0V6C (bisindolylmaleimide I)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology
MH  - Acetylcholine/*pharmacology
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Indoles/pharmacology
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Maleimides/pharmacology
MH  - Mutagenesis, Site-Directed
MH  - Oleic Acid/*pharmacology
MH  - Oocytes/*physiology
MH  - Point Mutation
MH  - Protein Kinase C/antagonists & inhibitors
MH  - Receptors, Nicotinic/biosynthesis/drug effects/*physiology
MH  - Recombinant Proteins/drug effects/metabolism
MH  - Serine
MH  - Torpedo
MH  - Xenopus laevis
EDAT- 1997/02/10 00:00
MHDA- 1997/02/10 00:01
CRDT- 1997/02/10 00:00
PHST- 1997/02/10 00:00 [pubmed]
PHST- 1997/02/10 00:01 [medline]
PHST- 1997/02/10 00:00 [entrez]
AID - 10.1097/00001756-199702100-00004 [doi]
PST - ppublish
SO  - Neuroreport. 1997 Feb 10;8(3):597-601. doi: 10.1097/00001756-199702100-00004.