PMID- 9107026
OWN - NLM
STAT- MEDLINE
DCOM- 19970701
LR  - 20190818
IS  - 0031-1820 (Print)
IS  - 0031-1820 (Linking)
VI  - 114 ( Pt 4)
DP  - 1997 Apr
TI  - Production of a monoclonal antibody specific for ovine immunoglobulin E and its 
      application to monitor serum IgE responses to Haemonchus contortus infection.
PG  - 395-406
AB  - Part of the C epsilon 3-C epsilon 4 region of the ovine immunoglobulin E (IgE) 
      gene (nucleotides 1111-1575) was amplified by PCR. The recombinant protein 
      (recIgE1-2) was expressed in E. coli and both monoclonal and polyclonal 
      antibodies were produced. These antibodies recognized recIgE1-2 and native IgE on 
      Western blots and in ELISA. The polyclonal serum showed cross-reactivity with 
      other sheep immunoglobulin classes. The monoclonal antibody was specific for 
      ovine IgE and goat IgE. Infection of sheep with the abomasal nematode Haemonchus 
      contortus resulted in elevated IgE levels in serum 2-4 weeks after infection, as 
      measured by sandwich ELISA using the rabbit polyclonal as capture antibody and 
      the monoclonal antibody against ovine IgE as second antibody. A negative 
      correlation between worm counts and total serum IgE levels at the end of the 
      experiment was found in repeatedly infected sheep. Significant increased levels 
      of excretory-secretory antigens specific IgE levels were found after H. contortus 
      infection. In contrast, no significant changes in 3rd-stage larvae (L3) 
      antigen-specific IgE titre in sera could be detected after infection.
FAU - Kooyman, F N
AU  - Kooyman FN
AD  - Department of Parasitology and Tropical Veterinary Medicine, Utrecht University, 
      The Netherlands.
FAU - Van Kooten, P J
AU  - Van Kooten PJ
FAU - Huntley, J F
AU  - Huntley JF
FAU - MacKellar, A
AU  - MacKellar A
FAU - Cornelissen, A W
AU  - Cornelissen AW
FAU - Schallig, H D
AU  - Schallig HD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*blood
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity
MH  - Cross Reactions
MH  - Duodenum/cytology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Haemonchiasis/immunology/*veterinary
MH  - Immunoglobulin E/*blood/genetics/immunology
MH  - Peptide Fragments/genetics/immunology
MH  - Recombinant Proteins/immunology
MH  - Sheep
MH  - Sheep Diseases/*immunology
MH  - Thymus Gland/cytology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1017/s0031182096008633 [doi]
PST - ppublish
SO  - Parasitology. 1997 Apr;114 ( Pt 4):395-406. doi: 10.1017/s0031182096008633.