PMID- 9107092
OWN - NLM
STAT- MEDLINE
DCOM- 19970604
LR  - 20071115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 82
IP  - 1
DP  - 1997 Jan-Feb
TI  - Detection of numerical aberrations in hematologic neoplasias by fluorescence in 
      situ hybridization.
PG  - 85-90
AB  - BACKGROUND AND OBJECTIVE: Over the last 5 years, fluorescence in situ 
      hybridization (FISH) techniques have had an important impact on molecular 
      cytogenetic diagnosis, providing a better understanding of the role of numerical 
      aberrations in hemopoietic neoplasms. The objective of this article is to analyze 
      the clinical applications of FISH in the management of hemopoietic malignancies. 
      EVIDENCE AND INFORMATION SOURCES: The material examined in the present review 
      includes articles and abstracts published in journals covered by the Science 
      Citation Index and Medline, and personal published and unpublished data. STATE OF 
      ART: FISH technology has the advantage of being relatively simple, fast and 
      flexible. Published data and ongoing prospective studies show that, under 
      well-controlled experimental conditions, interphase FISH is more sensitive than 
      conventional metaphase analysis in the detection of numerical abnormalities. Due 
      to the relatively high rate of false positive results, FISH cannot be used for 
      the study of minimal residual disease. However, since molecular strategies for 
      the detection of small-sized aneuploid clones have not been developed yet, FISH 
      represents a useful adjunct to conventional cytogenetics, especially for the 
      quantitation of the size of abnormal clones during the course of the disease and 
      to monitor XX/XY chimerism following sex mis-matched bone marrow transplantation. 
      Different approaches to the study of multiple cell-lineage involvement by 
      chromosome changes have been developed that take advantage of FISH techniques by: 
      a) simultaneous FISH and membrane immunophenotyping of cytologic and histologic 
      preparations; b) two-step analysis based on assessment of the morphology of cells 
      on panoptical stains, with subsequent hybridization and relocation of previously 
      identified cells; c) FISH analysis of enriched cell fractions obtained by cell 
      sorting or by separation of bone marrow cells on a density gradient, and d) study 
      of single hemopoietic colonies grown in semisolid media. PERSPECTIVES: New 
      molecular cytogenetic techniques, such as dual color FISH comparative genomic 
      hybridization, are at hand that will greatly improve the diagnostic power of 
      cytogenetics and make FISH increasingly useful in research laboratories as well 
      as in clinical practice.
FAU - Cuneo, A
AU  - Cuneo A
AD  - Dipartimento di Scienze Biomediche e Terapie Avanzate, Universita di Ferrara, 
      Italy.
FAU - Bigoni, R
AU  - Bigoni R
FAU - Roberti, M G
AU  - Roberti MG
FAU - Bardi, A
AU  - Bardi A
FAU - Balsamo, R
AU  - Balsamo R
FAU - Piva, N
AU  - Piva N
FAU - Castoldi, G
AU  - Castoldi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
MH  - *Aneuploidy
MH  - Bone Marrow Transplantation
MH  - Cell Lineage
MH  - Chimera
MH  - Chromosome Aberrations
MH  - Clone Cells/pathology
MH  - Female
MH  - Hematologic Neoplasms/*genetics/pathology/therapy
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Neoplasm, Residual
MH  - Neoplastic Stem Cells/pathology
MH  - Sensitivity and Specificity
RF  - 61
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1997 Jan-Feb;82(1):85-90.