PMID- 9109931
OWN - NLM
STAT- MEDLINE
DCOM- 19970501
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 94
IP  - 2
DP  - 1997 Apr
TI  - Bicolor fluorescence in situ hybridization on nuclei from formalin-fixed, 
      paraffin-embedded testicular germ cell tumors: comparison with standard metaphase 
      analysis.
PG  - 79-84
AB  - Rearrangements of chromosome 12, especially i(12p), are common in testicular germ 
      cell tumors (TGCT). We have developed 12p and 12q chromosome arm-specific 
      painting probes for fluorescence in situ hybridization (FISH) through the use of 
      chromosome microdissection. We developed a method to hybridize these probes to 
      interphase nuclei released from formalin-fixed, paraffin-embedded tumors (PET). 
      In this study, we compared simultaneous bicolor PET FISH painting with metaphase 
      chromosome analysis of fresh tissue from the same tumor. Bicolor PET FISH 
      produced patterns of 12p and 12q hybridization consistent with expectations based 
      on metaphase chromosome analysis; adjoined 12p and 12q regions appeared to detect 
      "normal" chromosome 12s, whereas relatively large isolated regions of 12p were 
      suggestive of i(12p), and small 12p regions probably represent cryptic 
      rearrangements of 12p. Fourteen tumors with successful cytogenetic analyses and 
      available archival material from the same tumor source were selected for study. 
      In a blinded analysis, PET FISH painting assessment was in very close agreement 
      with karyotypic findings in seven subjects, in close agreement in five, and 
      showed less agreement in two. Differences may be due in part to clonal selection 
      during culture for metaphase studies, or regional selection within the tumor. 
      Although PET FISH painting should not replace standard chromosome analysis, this 
      study shows that it can reliably predict chromosome 12 constitution in TGCT, can 
      serve as a useful adjunct to standard cytogenetics when such analysis is 
      unsuccessful, and can provide limited karyotyping of archival materials.
FAU - Blough, R I
AU  - Blough RI
AD  - Department of Medical and Molecular Genetics, Indiana University School of 
      Medicine, Indianapolis 46202-5251, USA.
FAU - Smolarek, T A
AU  - Smolarek TA
FAU - Ulbright, T M
AU  - Ulbright TM
FAU - Heerema, N A
AU  - Heerema NA
LA  - eng
GR  - R35 CA39844/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - Chromosome Aberrations/*diagnosis
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 12
MH  - DNA, Neoplasm/genetics
MH  - Fixatives
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping/*methods
MH  - Neoplasms, Germ Cell and Embryonal/diagnosis/*genetics
MH  - Paraffin
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0165-4608(96)00177-X [pii]
AID - 10.1016/s0165-4608(96)00177-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Apr;94(2):79-84. doi: 10.1016/s0165-4608(96)00177-x.