PMID- 9110368
OWN - NLM
STAT- MEDLINE
DCOM- 19970722
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 17
IP  - 3
DP  - 1997 Mar
TI  - Detection of cells of fetal origin from transcervical irrigations.
PG  - 243-8
AB  - Twenty-one women (age range 17-38 years) undergoing termination for social 
      reasons in the first trimester were selected. Ultrasound determined the gestation 
      and placental site. A commercially available flexible, sterile plastic tube was 
      advanced to the level of the internal os. Five to eight millilitres of normal 
      saline was injected and then gently aspirated into the tube. Chorionic villus 
      sampling (CVS) or amniocentesis was subsequently performed for back-up sexing. 
      Commercial X and Y probes were used simultaneously for fluorescence in situ 
      hybridization (FISH) study of the cervical washing and back-up samples, XY 
      signals were observed in 10 out of 12 male fetuses and 1 out of 4 female fetuses 
      (false positive), indicating 81.3 per cent correct prediction. Four cervical 
      washing samples did not provide enough cells for FISH. The frequency of cells 
      showing XY signals varied from 3.6 to 47.8 per cent in male-predicted 
      pregnancies. Placental-location, gestational age, or maternal age did not affect 
      the successful retrieval of fetal cells. Trophoblast cells were obtained in a 
      significant number (83 per cent) of pregnant women with male pregnancies by 
      transcervical irrigation in the first trimester. This preliminary part of the 
      study shows the presence of fetal cells in the transcervical samplings and the 
      feasibility of FISH in detecting them; however, further studies are needed to 
      isolate and purify fetal cells.
FAU - Daryani, Y P
AU  - Daryani YP
AD  - Department of Medical Genetics, St George's Hospital Medical School, London, U.K.
FAU - Penna, L K
AU  - Penna LK
FAU - Patton, M A
AU  - Patton MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Abortion, Induced
MH  - Adolescent
MH  - Adult
MH  - Amniocentesis
MH  - Cervix Uteri/*cytology
MH  - Chorionic Villi Sampling
MH  - Female
MH  - Fetus/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Sex Determination Analysis/*methods
MH  - Therapeutic Irrigation
EDAT- 1997/03/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0223(199703)17:3<243::AID-PD44>3.0.CO;2-E [pii]
AID - 10.1002/(sici)1097-0223(199703)17:3<243::aid-pd44>3.0.co;2-e [doi]
PST - ppublish
SO  - Prenat Diagn. 1997 Mar;17(3):243-8. doi: 
      10.1002/(sici)1097-0223(199703)17:3<243::aid-pd44>3.0.co;2-e.