PMID- 9111229
OWN - NLM
STAT- MEDLINE
DCOM- 19970508
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 45
IP  - 4
DP  - 1997 Apr
TI  - Intravital fluorescence microscopy: impact of light-induced phototoxicity on 
      adhesion of fluorescently labeled leukocytes.
PG  - 505-13
AB  - Alterations in leukocyte/endothelium interaction due to phototoxic effects of the 
      fluorescent dyes acridine orange (AO) and rhodamine 6G (Rh6G) were studied by 
      intravital microscopy using the dorsal skinfold model in awake Syrian golden 
      hamsters. AO (0.5 mg/kg/min; constant IV infusion) and Rh6G (0.1 micromol/kg; 
      bolus IV) were administered via an indwelling venous catheter. Five to seven 
      arterioles (35-55 microm) and postcapillary venules (30-65 microm) were 
      investigated in each animal. Vessels were exposed four times for 30 sec to 
      continuous light of the appropriate excitation wavelength with a 10-15-min time 
      interval between exposures. Animals were randomly assigned to five experimental 
      groups (five distinct light energy levels). AO and Rh6G induced leukocyte 
      rolling/sticking in postcapillary venules and arterioles when exposed to high 
      light energy levels. AO, but not Rh6G, induced arteriolar vasospasm when exposed 
      to high light energies. The potential phototoxic effect of AO and Rh6G is 
      demonstrated, as assessed by the stimulation of leukocyte-endothelium interaction 
      and arteriolar vasospasm in vivo. This study underscores the necessity to 
      optimize microscopic set-ups for intravital microscopy, to reduce the excitation 
      light energy level significantly, and to perform stringent control experiments, 
      ruling out an artificial phototoxicity-induced stimulation of leukocyte adhesion.
FAU - Saetzler, R K
AU  - Saetzler RK
AD  - Department of Physiology, Temple University, Philadelphia, Pennsylvania 19140, 
      USA.
FAU - Jallo, J
AU  - Jallo J
FAU - Lehr, H A
AU  - Lehr HA
FAU - Philips, C M
AU  - Philips CM
FAU - Vasthare, U
AU  - Vasthare U
FAU - Arfors, K E
AU  - Arfors KE
FAU - Tuma, R F
AU  - Tuma RF
LA  - eng
GR  - HL-46022/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Rhodamines)
RN  - 037VRW83CF (rhodamine 6G)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Animals
MH  - Arterioles/cytology/drug effects
MH  - Cell Adhesion/*radiation effects
MH  - Cricetinae
MH  - Endothelium, Vascular/cytology/drug effects
MH  - Female
MH  - Fluorescent Dyes
MH  - Leukocytes/cytology/*radiation effects
MH  - *Light
MH  - Mesocricetus
MH  - Microscopy, Fluorescence
MH  - Rhodamines/pharmacology
MH  - Vasoconstriction/drug effects
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1177/002215549704500403 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1997 Apr;45(4):505-13. doi: 10.1177/002215549704500403.