PMID- 9111471
OWN - NLM
STAT- MEDLINE
DCOM- 19970506
LR  - 20191024
IS  - 1077-9450 (Print)
IS  - 1077-9450 (Linking)
VI  - 14
IP  - 4
DP  - 1997 Apr 1
TI  - Primary proliferative responses to peptides of HIV Gag p24.
PG  - 301-6
AB  - Primary proliferative responses can be initiated by adding dendritic cells pulsed 
      with antigen to autologous T cells in 20-microliter hanging drop cultures. To 
      identify primary T-cell epitopes of HIV gag, a series of 23 overlapping peptides, 
      15 amino acids long, spanning the p24 region were used. Significant proliferative 
      responses were induced in cells from healthy HIV-negative donors by 11 of these 
      peptides. One of two peptides that bound human leukocyte antigen (HLA)-A *0201 in 
      a peptide-binding assay using the antigen-processing defective cell line T2 also 
      induced a primary proliferative response. Primary T-cell proliferation was seen 
      in response to some peptides of gag that have not previously been identified as 
      T-cell epitopes in cells from infected individuals. These epitopes might be 
      useful not only for vaccines in antigenically naive individuals but also might 
      increase the breadth of immune responses in seropositive patients.
FAU - Bedford, P A
AU  - Bedford PA
AD  - Antigen Presentation Research Group, Imperial College School of Medicine, 
      Northwick Park Institute for Medical Research, Harrow, England, U.K.
FAU - Clarke, L B
AU  - Clarke LB
FAU - Hastings, G Z
AU  - Hastings GZ
FAU - Knight, S C
AU  - Knight SC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Acquir Immune Defic Syndr Hum Retrovirol
JT  - Journal of acquired immune deficiency syndromes and human retrovirology : 
      official publication of the International Retrovirology Association
JID - 9501482
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - HIV Core Protein p24/chemistry/*immunology
MH  - HIV Seronegativity
MH  - Humans
MH  - In Vitro Techniques
MH  - *Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - Peptides/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1097/00042560-199704010-00001 [doi]
PST - ppublish
SO  - J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Apr 1;14(4):301-6. doi: 
      10.1097/00042560-199704010-00001.