PMID- 9111518
OWN - NLM
STAT- MEDLINE
DCOM- 19970509
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 76
IP  - 4
DP  - 1997 Apr
TI  - The pattern of monocyte recruitment in tumors is modulated by MCP-1 expression 
      and influences the rate of tumor growth.
PG  - 579-90
AB  - Macrophage infiltration is an important aspect of the host response to tumor 
      growth. Tumor-associated macrophages often exhibit intratumoral or peritumoral 
      infiltration patterns. However, the mechanism that mediates distinctive patterns 
      of macrophage infiltration and their impact on tumor growth is not known. We 
      studied macrophage infiltration and the effect of this infiltration on tumor 
      growth in mice inoculated with four human tumor cell lines. We report here that 
      tumor cells producing high levels of monocyte chemoattractant protein-1 (MCP-1) 
      had earlier recruitment of intratumoral macrophages than did lower producers and 
      nonproducers. These MCP-1 high producers also had a lower tumor take rate than 
      tumors that produced little or no MCP-1. The importance of early recruitment of 
      monocytes was further demonstrated by introduction of fMLP (a chemotactic 
      peptide) to the tumor sites. Administration of fMLP abolished the tumor formation 
      when applied at the time of tumor-cell inoculation. When injection of fMLP was 
      delayed, tumor growth was slowed but not eliminated. We also demonstrated that 
      all MCP-1-producing tumors exhibited both intratumoral and peritumoral macrophage 
      patterns, whereas MCP-1 nonproducers exhibited a peritumoral macrophage pattern 
      only. The number of intratumoral macrophages was negatively correlated with tumor 
      size. Collectively, our results suggest that (a) early recruitment of monocytes 
      inhibits tumor growth; and (b) early intratumoral macrophage infiltration in vivo 
      corresponds to the level of MCP-1 produced by tumors in vitro. In contrast, 
      recruitment of neutrophils was not negatively correlated with tumor growth. Thus, 
      early recruitment of macrophages may be beneficial to cancer patients.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Pathology and Laboratory Medicine, Boston University School of 
      Medicine, Massachusetts, USA.
FAU - Khayat, A
AU  - Khayat A
FAU - Cheng, H
AU  - Cheng H
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - DE-07559/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Chemokine CCL2)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Monocytes/*physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neoplasms, Experimental/drug therapy/*metabolism/*pathology
MH  - Neutrophils/physiology
MH  - Tumor Cells, Cultured
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1997 Apr;76(4):579-90.