PMID- 9111994
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20190816
IS  - 0009-9163 (Print)
IS  - 0009-9163 (Linking)
VI  - 51
IP  - 2
DP  - 1997 Feb
TI  - Centromeric alphoid DNA heteromorphisms of chromosome 21 revealed by 
      FISH-technique.
PG  - 91-3
AB  - The centromeric heterochromatin of chromosome 21 has been evaluated by the 
      fluorescence in situ hybridization (FISH) technique. It was found that the 
      alphoid DNA sequences of pericentromeric regions of chromosome 21 were highly 
      heteromorphic when a centromeric specific probe was hybridized to these 
      sequences. The variations were so extreme that they could even be arbitrarily 
      classified into at least five sizes by comparison with the length of the short 
      arm (p) of chromosome 18. They are negative (1); small (2); medium (3); large 
      (4); and very large (5). We used 15 normal cases and 12 individuals with trisomy 
      21 (Down syndrome), and the incidences for these five classes were 3.0%, 22.7%, 
      59.2%, 13.6% and 1.5%, respectively. At least 3% of the chromosomes no. 21 did 
      not show any trace of hybridization signals, which apparently escape detection at 
      interphase level as well. Although, the variations observed in the present study 
      are continuous, the proposed classification may yield some implications for 
      future investigations.
FAU - Verma, R S
AU  - Verma RS
AD  - Division of Genetics, Long Island College Hospital, SUNY Health Science Center, 
      Brooklyn, USA.
FAU - Batish, S D
AU  - Batish SD
FAU - Gogineni, S K
AU  - Gogineni SK
FAU - Kleyman, S M
AU  - Kleyman SM
FAU - Stetka, D G
AU  - Stetka DG
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Clin Genet
JT  - Clinical genetics
JID - 0253664
RN  - 0 (Chromatin)
SB  - IM
CIN - Clin Genet. 1998 Mar;53(3):231-2. PMID: 9630084
MH  - Centromere/*genetics
MH  - Chromatin/*genetics
MH  - *Chromosomes, Human, Pair 21
MH  - Down Syndrome/genetics
MH  - *Genetic Variation
MH  - Genetics, Population
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1111/j.1399-0004.1997.tb02426.x [doi]
PST - ppublish
SO  - Clin Genet. 1997 Feb;51(2):91-3. doi: 10.1111/j.1399-0004.1997.tb02426.x.