PMID- 9112394
OWN - NLM
STAT- MEDLINE
DCOM- 19970509
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 5
DP  - 1997 May
TI  - Tumor necrosis factor-alpha-stimulated lactate production is linked to lactate 
      dehydrogenase A expression and activity increase in porcine cultured Sertoli 
      cells.
PG  - 1964-71
AB  - By using, as a model, cultured testicular immature Sertoli cells, the action of 
      tumor necrosis factor-alpha (TNF alpha) and the site of action of the cytokine on 
      lactate production were studied. TNF alpha stimulated in a time- and 
      dose-dependent manner (with an ED50 of 0.1 nM) Sertoli cell lactate production. 
      Two major sites involved in TNF alpha action were identified. Firstly, TNF alpha 
      was shown to increase the uptake of glucose substrate in a time- and 
      dose-dependent manner. The maximal effect was observed after 24 h of treatment, 
      with an ED50 of 0.1 nM. Secondly, TNF alpha increased the activity of lactate 
      dehydrogenase (LDH) A isoform, which is involved in the conversion of pyruvate 
      into lactate. This increase in LDH-A activity was detected at 12 h and was 
      maximal after 24 h of treatment with TNF alpha. The stimulatory effect of the 
      cytokine on the LDH-A isoform was observed with an ED50 of 0.05 nM. Such an 
      increase in LDH-A activity was related to an increase in LDH-A expression, 
      because TNF alpha stimulated LDH-A messenger RNA (size, 1.5 kilobases, determined 
      by Northern blotting analysis). Together, assuming that in the seminiferous 
      tubules, TNF alpha is produced by spermatids that use lactate for their energetic 
      metabolism, we suggest that the cytokine may potentially represent a signal used 
      by germ cells to enhance lactate production in Sertoli cells through, at least, a 
      redistribution of LDH isoforms in favor of LDH-A.
FAU - Nehar, D
AU  - Nehar D
AD  - INSERM U-407, Communications Cellulaires en Biologie de la Reproduction, Centre 
      Hospitalier Lyon-Sud, Pierre-Benite, France.
FAU - Mauduit, C
AU  - Mauduit C
FAU - Boussouar, F
AU  - Boussouar F
FAU - Benahmed, M
AU  - Benahmed M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Electrophoresis, Agar Gel
MH  - Gene Expression
MH  - Glucose/metabolism
MH  - Isoenzymes
MH  - L-Lactate Dehydrogenase/genetics/isolation & purification/*metabolism
MH  - Lactic Acid/*biosynthesis
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Sertoli Cells/*metabolism
MH  - Swine
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1210/endo.138.5.5098 [doi]
PST - ppublish
SO  - Endocrinology. 1997 May;138(5):1964-71. doi: 10.1210/endo.138.5.5098.