PMID- 9112975
OWN - NLM
STAT- MEDLINE
DCOM- 19970502
LR  - 20151119
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 38
IP  - 5
DP  - 1997 Apr
TI  - Prednisolone, platelet-activating factor receptor antagonist, or superoxide 
      dismutase reduced leukocyte entrapment induced by interferon alpha in retinal 
      microcirculation.
PG  - 811-6
AB  - PURPOSE: Interferon (IFN) alpha has been suggested as a possible treatment for 
      choroidal neovascularization. However, the pathogenesis of retinal complications 
      after IFN therapy still is unknown. Previously, we have shown that IFN alpha 
      induced leukocyte entrapment in retinal microcirculation. The current study was 
      designed to determine if leukocyte entrapment can be reduced by the agents that 
      modulate leukocyte-endothelial adherence. METHODS: Interferon alpha was 
      administered intravenously in rats. Simultaneously, prednisolone (PSL), 
      platelet-activating factor receptor antagonist (CV-6209), or superoxide dismutase 
      (SOD) was given to the rats. Leukocyte dynamics were observed with acridine 
      orange (AO) digital fluorography, which uses a nuclear fluorescent dye of AO and 
      scanning laser ophthalmoscopy. The number of trapped leukocytes in each group was 
      assessed with a personal computer-based image analysis. RESULTS: Interferon alpha 
      induced leukocyte entrapment in retinal microcirculation and increased leukocyte 
      adherence to the vessel walls. The simultaneous administration of PSL, CV-6209, 
      or SOD inhibited leukocyte adherence to the venous walls and significantly 
      reduced the number of trapped leukocytes. CONCLUSIONS: Acridine orange digital 
      fluorography was helpful to quantitate leukocyte-endothelial interactions in 
      retinal microcirculation. The results suggested that increased leukocyte 
      adherence after IFN alpha administration was reduced significantly by PSL, 
      CV-6209, or SOD. These agents may be useful to prevent IFN-induced 
      microcirculatory disturbances.
FAU - Nishiwaki, H
AU  - Nishiwaki H
AD  - Department of Ophthalmology and Visual Science, Graduate School of Medicine, 
      Kyoto University, Japan.
FAU - Ogura, Y
AU  - Ogura Y
FAU - Miyamoto, K
AU  - Miyamoto K
FAU - Hiroshiba, N
AU  - Hiroshiba N
FAU - Hamada, M
AU  - Hamada M
FAU - Honda, Y
AU  - Honda Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antiviral Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glucocorticoids)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Pyridinium Compounds)
RN  - 100488-87-7 (CV 6209)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Antiviral Agents
MH  - Capillaries/cytology
MH  - Cell Adhesion/*drug effects
MH  - Cell Movement
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Fluorescein Angiography
MH  - Fluorescent Dyes
MH  - Fluorophotometry
MH  - Glucocorticoids/*pharmacology
MH  - Leukocytes/cytology/*metabolism
MH  - Microcirculation
MH  - Platelet Activating Factor/*antagonists & inhibitors
MH  - Prednisolone/*pharmacology
MH  - Pyridinium Compounds/*pharmacology
MH  - Rats
MH  - Retinal Vessels/*metabolism
MH  - Superoxide Dismutase/*pharmacology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1997 Apr;38(5):811-6.