PMID- 9115992
OWN - NLM
STAT- MEDLINE
DCOM- 19970421
LR  - 20131121
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 36
IP  - 11
DP  - 1997 Mar 18
TI  - The P4-P6 domain directs higher order folding of the Tetrahymena ribozyme core.
PG  - 3159-69
AB  - The active site of group I self-splicing introns occurs at the interface of two 
      proposed structural domains. In the Tetrahymena intron, half of the catalytic 
      core resides within the independently-folding P4-P6 domain while the other half 
      belongs to a putative domain that includes helices P3, P7, P8, and P9 (P3-P9). To 
      determine whether the P3-P9 region of the intron can also fold independently, we 
      used Fe(II)-EDTA and dimethyl sulfate to probe the solvent accessibility of 
      separate fragments of the Tetrahymena intron. These RNAs self-assemble into an 
      active complex in trans, enabling analysis of their structural features both 
      alone and within the complex. Our results show that while the P3-P9 region of the 
      intron retains its secondary structure, most of the tertiary interactions within 
      this region do not form stably in the absence of the P4-P6 domain. This indicates 
      that the P4-P6 domain induces folding in the P3-P9 region, organizing the 
      catalytic cleft between them. Thus the P4-P6 domain provides a scaffold for the 
      folding of the Tetrahymena intron core.
FAU - Doherty, E A
AU  - Doherty EA
AD  - Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, 
      Connecticut 06520, USA.
FAU - Doudna, J A
AU  - Doudna JA
LA  - eng
GR  - GM 07223/GM/NIGMS NIH HHS/United States
GR  - GM 22778-21/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Ferrous Compounds)
RN  - 0 (RNA, Catalytic)
RN  - 0 (RNA, Protozoan)
RN  - 0 (Solvents)
RN  - 0 (Sulfuric Acid Esters)
RN  - 15651-72-6 (Fe(II)-EDTA)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - JW5CW40Z50 (dimethyl sulfate)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Edetic Acid
MH  - Ferrous Compounds
MH  - Genes, Protozoan
MH  - Introns
MH  - Models, Structural
MH  - Molecular Sequence Data
MH  - *Nucleic Acid Conformation
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - RNA, Catalytic/*chemistry/isolation & purification/metabolism
MH  - RNA, Protozoan/chemistry/isolation & purification/metabolism
MH  - Solvents
MH  - Sulfuric Acid Esters
MH  - Templates, Genetic
MH  - Tetrahymena/*genetics
EDAT- 1997/03/18 00:00
MHDA- 1997/03/18 00:01
CRDT- 1997/03/18 00:00
PHST- 1997/03/18 00:00 [pubmed]
PHST- 1997/03/18 00:01 [medline]
PHST- 1997/03/18 00:00 [entrez]
AID - bi962428+ [pii]
AID - 10.1021/bi962428+ [doi]
PST - ppublish
SO  - Biochemistry. 1997 Mar 18;36(11):3159-69. doi: 10.1021/bi962428+.