PMID- 9116278
OWN - NLM
STAT- MEDLINE
DCOM- 19970422
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 89
IP  - 7
DP  - 1997 Apr 1
TI  - Frequency of fetal cells in sorted subpopulations of nucleated erythroid and 
      CD34+ hematopoietic progenitor cells from maternal peripheral blood.
PG  - 2347-58
AB  - Fetal cells that circulate in maternal peripheral blood (PB) during pregnancy 
      offer a potential source of nucleated fetal material for noninvasive prenatal 
      diagnosis. Fluorescence-activated cell sorting was used to target two populations 
      of fetal cells: nucleated erythroid cells (NECs; CD71/glycophorin-A+ CD45(lo-int) 
      CD34-) and hematopoietic progenitor cells (CD34+ cells; CD34++ 
      CD71/glycophorin-A- CD45(int)). Fetal cells were detected by fluorescence in situ 
      hybridization (FISH) using directly conjugated chromosome X and Y probes in 65% 
      (13 of 20) of the maternal PBs (fetal karyotype 46,XY). The frequency of fetal 
      cells isolated from the NEC and CD34+ fractions was, respectively, 0 to 14 and 0 
      to 7 cells per 2 x 10(7) previously frozen maternal cells (approximately 20 mL of 
      blood). In nonfrozen samples, the yield and recovery of fetal cells was 
      moderately improved. Culturing the CD34+ sorted fractions in serum-free media 
      with cytokines improved the quality of the FISH preparations and resulted in a 
      slight expansion in detectable fetal cells. The frequency of fetal cells isolated 
      from cultured CD34+ fractions was 0 to 35 and 0 to 93 cells per 2 x 10(7) 
      previously frozen and nonfrozen maternal PB cells, respectively. These results 
      document the isolation, characterization, and enumeration of fetal cells from the 
      maternal periphery that appear to be present in most, but not all, samples 
      analyzed.
FAU - Little, M T
AU  - Little MT
AD  - Department of Medical Genetics, University of British Columbia, Vancouver, 
      Canada.
FAU - Langlois, S
AU  - Langlois S
FAU - Wilson, R D
AU  - Wilson RD
FAU - Lansdorp, P M
AU  - Lansdorp PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Antigens, CD34/*analysis
MH  - *Blood Cell Count
MH  - Cell Nucleus
MH  - *Erythroid Precursor Cells
MH  - Female
MH  - *Fetomaternal Transfusion
MH  - Fetus/*cytology
MH  - *Hematopoietic Stem Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Pregnancy
MH  - Prenatal Diagnosis
MH  - Sensitivity and Specificity
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0006-4971(20)59042-3 [pii]
PST - ppublish
SO  - Blood. 1997 Apr 1;89(7):2347-58.