PMID- 9116616
OWN - NLM
STAT- MEDLINE
DCOM- 19970422
LR  - 20041117
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 19
IP  - 2
DP  - 1997 Jan
TI  - Successful engraftment after primary graft failure in aplastic anemia using G-CSF 
      mobilized peripheral stem cell transfusions.
PG  - 175-7
AB  - A 19-year-old male underwent allogeneic BMT for severe aplastic anaemia (SAA) 
      from his HLA- and blood group-identical sister. He was conditioned with 
      cyclophosphamide (CY) and single fraction total lymphoid irradiation (TLI). GVHD 
      prophylaxis consisted of FK506 and a short course of methotrexate. The patient 
      failed to achieve durable trilineage hematopoietic engraftment. There was no 
      significant myeloid response to GM-CSF or G-CSF. Evaluation of FACS-sorted 
      peripheral T cells from the patient by fluorescence in situ hybridization (FISH) 
      revealed mixed chimerism (44% host origin). Fifty-three days after the first BMT, 
      he was treated with G-CSF primed, unmanipulated PBSC transfusions (5.28 x 
      10(8)/kg mononuclear, 4.28 x 10(6)/kg CD34+, 292.51 x 10(6)/kg CD3+ cells) from 
      his original donor without reconditioning. FK506 was continued at the same dose. 
      Neutrophil recovery to 0.5 x 10(9)/l and platelet engraftment to 20 x 10(9)/l was 
      achieved 11 and 27 days following the first dose of allogeneic PBSC transfusion, 
      respectively. On day 23 a repeat FISH on the patient's sorted peripheral T 
      lymphocytes revealed 91% donor origin T cells. The patient is currently well with 
      a stable engraftment 6 months following allogeneic PBSC transfusion, with no 
      signs of acute of chronic GVHD.
FAU - Redei, I
AU  - Redei I
AD  - Emory University Bone Marrow Transplant and Leukemia Program, Atlanta, GA, USA.
FAU - Waller, E K
AU  - Waller EK
FAU - Holland, H K
AU  - Holland HK
FAU - Devine, S M
AU  - Devine SM
FAU - Wingard, J R
AU  - Wingard JR
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Anemia, Aplastic/*therapy
MH  - Cell Separation
MH  - Graft Rejection
MH  - Graft Survival
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*drug effects/pathology
MH  - Humans
MH  - Male
MH  - Transplantation, Homologous
EDAT- 1997/01/02 00:00
MHDA- 1997/01/02 00:01
CRDT- 1997/01/02 00:00
PHST- 1997/01/02 00:00 [pubmed]
PHST- 1997/01/02 00:01 [medline]
PHST- 1997/01/02 00:00 [entrez]
AID - 10.1038/sj.bmt.1700623 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 1997 Jan;19(2):175-7. doi: 10.1038/sj.bmt.1700623.