PMID- 9117038
OWN - NLM
STAT- MEDLINE
DCOM- 19970418
LR  - 20220317
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 155
IP  - 3
DP  - 1997 Mar
TI  - Mycobacterium tuberculosis enhances human immunodeficiency virus-1 replication in 
      the lung.
PG  - 996-1003
AB  - We investigated the in vivo effect of coinfection of Mycobacterium tuberculosis 
      on human immunodeficiency virus type 1 (HIV-1) replication using bronchoalveolar 
      lavage (BAL) of 11 HIV-1-infected patients with pulmonary tuberculosis and 10 
      patients with no lung disease. Lung segments involved with pulmonary tuberculosis 
      had significantly elevated HIV-1 branched DNA (bDNA) levels and p24 in BAL 
      compared with lung segments uninvolved with tuberculosis or with BAL from 
      patients with no lung disease. The BAL viral burden was higher than plasma HIV-1 
      in tuberculosis patients, indicating local production of virus. BAL HIV-1 bDNA 
      declined over the course of treatment for tuberculosis in three patients who 
      underwent serial bronchoscopies. Tumor necrosis factor-alpha (TNF-alpha) and 
      HIV-1 bDNA particles were strongly correlated (r2 = 0.9, p < 0.01) in lung 
      segments involved with tuberculosis. The deduced amino acid sequence of HIV-1 
      gp120 V3 region from involved segments of three patients with pulmonary 
      tuberculosis showed basic substitutions associated with altered viral phenotype. 
      Phylogenetic analysis of V3 sequences demonstrated that BAL HIV-1 RNA had 
      diverged from plasma. These data support the conclusion that pulmonary 
      tuberculosis enhances local HIV-1 replication in vivo.
FAU - Nakata, K
AU  - Nakata K
AD  - Division of Pulmonary and Critical Care Medicine, New York University Medical 
      Center, New York, USA.
FAU - Rom, W N
AU  - Rom WN
FAU - Honda, Y
AU  - Honda Y
FAU - Condos, R
AU  - Condos R
FAU - Kanegasaki, S
AU  - Kanegasaki S
FAU - Cao, Y
AU  - Cao Y
FAU - Weiden, M
AU  - Weiden M
LA  - eng
GR  - HL51474/HL/NHLBI NIH HHS/United States
GR  - HL51494/HL/NHLBI NIH HHS/United States
GR  - MO1 RR00096/RR/NCRR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (DNA, Viral)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Viral)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - DNA, Viral/analysis
MH  - HIV Envelope Protein gp120/genetics
MH  - HIV Infections/blood/*complications/virology
MH  - HIV-1/genetics/*physiology
MH  - Humans
MH  - Interleukin-6/analysis
MH  - Lung/*virology
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/analysis
MH  - Tuberculosis, Pulmonary/blood/*complications/virology
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - *Virus Replication
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1164/ajrccm.155.3.9117038 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1997 Mar;155(3):996-1003. doi: 
      10.1164/ajrccm.155.3.9117038.