PMID- 9118773
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20220330
IS  - 0149-5992 (Print)
IS  - 0149-5992 (Linking)
VI  - 20
IP  - 2
DP  - 1997 Feb
TI  - Total radical-trapping antioxidant parameter in NIDDM patients.
PG  - 194-7
AB  - OBJECTIVE: The existence of an oxidative stress in diabetes is still debated. 
      This is largely due to the lack of good tools to assay the level of oxidative 
      stress. The use of total radical-trapping antioxidant parameter (TRAP) has 
      recently been proposed to explore the antioxidant property of a plasma sample. 
      TRAP may be either directly measured by a fluorescence-based method (TRAPm) or 
      calculated (TRAPc) by a mathematical formula, taking into account the serum 
      levels of four natural antioxidants: protein-bound SH (thiol) groups, uric acid, 
      vitamin E, and vitamin C. The difference between TRAPm and TRAPc is due to 
      antioxidants, which are still unidentified, and to the possible synergism among 
      the antioxidants. RESEARCH DESIGN AND METHODS: In this study, we evaluated 
      malondialdehyde (MDA), TRAPm, TRAPc, protein-bound SH groups, uric acid, vitamin 
      E, and vitamin C in 40 NIDDM patients and 40 matched normal control subjects. 
      RESULTS: TRAPm and TRAPc were significantly lower in diabetic patients. A good 
      correlation between TRAPm and TRAPc was found in both NIDDM patients (r = 0.68, P 
      < 0.0001) and control subjects (r = 0.74, P < 0.0001). Protein-bound SH groups 
      and uric acid were significantly lower in diabetic subjects, while MDA and 
      vitamin E level were significantly higher. After correction for serum 
      triglycerides (MDA) and cholesterol (vitamin E), MDA lost significance, while 
      vitamin E did not. Vitamin C was not different in the two groups. CONCLUSIONS: 
      These data show decreased TRAP levels in NIDDM patients, suggesting the existence 
      of lower antioxidant defenses in diabetes. The decrease appears to be due to 
      various antioxidants, some of them not yet clearly defined. TRAP may represent a 
      more reliable estimation of serum antioxidant capacity than the measurement of 
      each known antioxidants. The correlation found between TRAPm and TRAPc values 
      suggests that TRAPc, easier to measure than TRAPm, might be adequately reliable 
      for routine assessment of oxidative stress in diabetic patients.
FAU - Ceriello, A
AU  - Ceriello A
AD  - Department of Medicine and Pathology, Clinical and Experimental, University of 
      Udine, Italy.
FAU - Bortolotti, N
AU  - Bortolotti N
FAU - Falleti, E
AU  - Falleti E
FAU - Taboga, C
AU  - Taboga C
FAU - Tonutti, L
AU  - Tonutti L
FAU - Crescentini, A
AU  - Crescentini A
FAU - Motz, E
AU  - Motz E
FAU - Lizzio, S
AU  - Lizzio S
FAU - Russo, A
AU  - Russo A
FAU - Bartoli, E
AU  - Bartoli E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diabetes Care
JT  - Diabetes care
JID - 7805975
RN  - 0 (Antioxidants)
RN  - 0 (Free Radicals)
RN  - 0 (Sulfhydryl Compounds)
RN  - 1406-18-4 (Vitamin E)
RN  - 268B43MJ25 (Uric Acid)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Antioxidants/*analysis
MH  - Ascorbic Acid/blood
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Female
MH  - Free Radicals/blood
MH  - Humans
MH  - Male
MH  - Malondialdehyde/blood
MH  - Middle Aged
MH  - Oxidative Stress
MH  - Sulfhydryl Compounds/blood
MH  - Uric Acid/blood
MH  - Vitamin E/blood
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.2337/diacare.20.2.194 [doi]
PST - ppublish
SO  - Diabetes Care. 1997 Feb;20(2):194-7. doi: 10.2337/diacare.20.2.194.