PMID- 9119087
OWN - NLM
STAT- MEDLINE
DCOM- 19970418
LR  - 20220309
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 404
IP  - 2-3
DP  - 1997 Mar 10
TI  - Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and 
      ectopic bone formation.
PG  - 319-23
AB  - We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation 
      and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic 
      cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased 
      by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding 
      amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected 
      chicken fibroblast cultures also significantly increased ALP activity in both 
      C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of 
      Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone 
      formation. These results indicate that SHH induces osteoblast differentiation and 
      ectopic bone formation.
FAU - Kinto, N
AU  - Kinto N
AD  - Department of Oral Pathology, School of Dentistry, Showa University, 
      Shinagawa-ku, Tokyo, Japan.
FAU - Iwamoto, M
AU  - Iwamoto M
FAU - Enomoto-Iwamoto, M
AU  - Enomoto-Iwamoto M
FAU - Noji, S
AU  - Noji S
FAU - Ohuchi, H
AU  - Ohuchi H
FAU - Yoshioka, H
AU  - Yoshioka H
FAU - Kataoka, H
AU  - Kataoka H
FAU - Wada, Y
AU  - Wada Y
FAU - Yuhao, G
AU  - Yuhao G
FAU - Takahashi, H E
AU  - Takahashi HE
FAU - Yoshiki, S
AU  - Yoshiki S
FAU - Yamaguchi, A
AU  - Yamaguchi A
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - *Bone Development
MH  - Cell Differentiation
MH  - Cell Line
MH  - Chick Embryo
MH  - Coculture Techniques
MH  - Embryonic Induction
MH  - Fibroblasts/cytology/metabolism/transplantation
MH  - Hedgehog Proteins
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Nude
MH  - Osteoblasts/*cytology/transplantation
MH  - Peptide Fragments/biosynthesis
MH  - *Protein Biosynthesis
MH  - Recombinant Proteins/biosynthesis
MH  - Time Factors
MH  - *Trans-Activators
MH  - Transfection
EDAT- 1997/03/10 00:00
MHDA- 1997/03/10 00:01
CRDT- 1997/03/10 00:00
PHST- 1997/03/10 00:00 [pubmed]
PHST- 1997/03/10 00:01 [medline]
PHST- 1997/03/10 00:00 [entrez]
AID - S0014-5793(97)00014-8 [pii]
AID - 10.1016/s0014-5793(97)00014-8 [doi]
PST - ppublish
SO  - FEBS Lett. 1997 Mar 10;404(2-3):319-23. doi: 10.1016/s0014-5793(97)00014-8.