PMID- 9119371
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20190905
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 39
IP  - 3
DP  - 1997 Feb 1
TI  - A 5.5-Mb high-resolution integrated map of distal 11q13.
PG  - 340-7
AB  - The distal part of 11q13, which contains several genes relevant to human 
      diseases, has been poorly mapped as part of genome-wide mapping efforts. In the 
      prospect of drawing a fine-scale integrated map of the area containing KRN1 and 
      OMP, we have established a framework of markers by hybridization to DNA of 
      somatic cell hybrids and by fluorescence in situ hybridization (FISH) on 
      metaphase chromosomes. The probes studied were used to isolate 27 YACs and 16 
      cosmids that could be organized in three contigs covering approximately 6 Mb. 
      These contigs were separated by two gaps that are likely to contain sequences 
      underrepresented in YAC libraries. They were then integrated based on long-range 
      restriction mapping and DNA-fiber FISH into a high-resolution physical map, which 
      covers a 5.5-Mb region and includes 36 anonymous markers and 10 genes. This map 
      will be used to search for genes within the 2/3 of this region where none have 
      been localized as yet. It will also lay the ground for the characterization of an 
      amplicon surrounding GARP in breast cancer and for the search of disease genes 
      within this region.
FAU - Merscher, S
AU  - Merscher S
AD  - Laboratoire de Genetique Moleculaire des Cancers Humains, URA CNRS 1462, 
      Universite de Nice-Sophia Antipolis, France.
FAU - Bekri, S
AU  - Bekri S
FAU - de Leeuw, B
AU  - de Leeuw B
FAU - Pedeutour, F
AU  - Pedeutour F
FAU - Grosgeorge, J
AU  - Grosgeorge J
FAU - Shows, T B
AU  - Shows TB
FAU - Mullenbach, R
AU  - Mullenbach R
FAU - Le Paslier, D
AU  - Le Paslier D
FAU - Nowak, N J
AU  - Nowak NJ
FAU - Gaudray, P
AU  - Gaudray P
LA  - eng
GR  - EY10541/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 11
MH  - DNA Probes
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Restriction Mapping
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0888754396944602 [pii]
AID - 10.1006/geno.1996.4460 [doi]
PST - ppublish
SO  - Genomics. 1997 Feb 1;39(3):340-7. doi: 10.1006/geno.1996.4460.