PMID- 9120011
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 99
IP  - 7
DP  - 1997 Apr 1
TI  - Conversion of the major birch pollen allergen, Bet v 1, into two nonanaphylactic 
      T cell epitope-containing fragments: candidates for a novel form of specific 
      immunotherapy.
PG  - 1673-81
AB  - A novel approach to reduce the anaphylactic activity of allergens is suggested. 
      The strategy makes use of the presence of conformational immunoglobulin E (IgE) 
      epitopes on one of the most common allergens. The three dimensional structure of 
      the major birch pollen allergen, Bet v 1, was disrupted by expressing two parts 
      of the Bet v 1 cDNA representing amino acids 1-74 and 75-160 in Escherichia coli. 
      In contrast to the complete recombinant Bet v 1, the fragments showed almost no 
      allergenicity and exhibited random coil conformation as analyzed by circular 
      dichroism. Both nonanaphylactic fragments induced proliferation of human Bet v 
      1-specific T cell clones, indicating that they harbored all dominant T cell 
      epitopes and therefore may be considered as a basis for the development of a safe 
      and specific T cell immunotherapy.
FAU - Vrtala, S
AU  - Vrtala S
AD  - Department of Immunopathology, Institute of General and Experimental Pathology, 
      AKH, University of Vienna, Austria.
FAU - Hirtenlehner, K
AU  - Hirtenlehner K
FAU - Vangelista, L
AU  - Vangelista L
FAU - Pastore, A
AU  - Pastore A
FAU - Eichler, H G
AU  - Eichler HG
FAU - Sperr, W R
AU  - Sperr WR
FAU - Valent, P
AU  - Valent P
FAU - Ebner, C
AU  - Ebner C
FAU - Kraft, D
AU  - Kraft D
FAU - Valenta, R
AU  - Valenta R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 126161-14-6 (Bet v 1 allergen, Betula)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Allergens
MH  - Anaphylaxis/prevention & control
MH  - Animals
MH  - Antigens, Plant
MH  - Circular Dichroism
MH  - *Epitopes
MH  - Histamine Release
MH  - Humans
MH  - Hypersensitivity/*therapy
MH  - Immunoglobulin E/metabolism
MH  - Immunotherapy
MH  - Mice
MH  - Peptide Fragments/*immunology
MH  - Plant Proteins/*chemistry/immunology/isolation & purification
MH  - Protein Conformation
MH  - Recombinant Proteins/isolation & purification
MH  - T-Lymphocytes/*immunology
PMC - PMC507987
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
PMCR- 1997/04/01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PHST- 1997/04/01 00:00 [pmc-release]
AID - 10.1172/JCI119330 [doi]
PST - ppublish
SO  - J Clin Invest. 1997 Apr 1;99(7):1673-81. doi: 10.1172/JCI119330.