PMID- 9121423
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 17
IP  - 4
DP  - 1997 Apr
TI  - In vivo antisense oligodeoxynucleotide mapping reveals masked regulatory elements 
      in an mRNA dormant in mouse oocytes.
PG  - 1759-67
AB  - In mouse oocytes, tissue-type plasminogen activator (tPA) mRNA is under 
      translational control. The newly transcribed mRNA undergoes deadenylation and 
      translational silencing in growing oocytes, while readenylation and translation 
      occur during meiotic maturation. To localize regulatory elements controlling tPA 
      mRNA expression, we identified regions of the endogenous transcript protected 
      from hybridization with injected antisense oligodeoxynucleotides. Most of the 
      targeted sequences in either the 5' untranslated region (5'UTR), coding region, 
      or 3'UTR were accessible to hybridization, as revealed by inhibition of tPA 
      synthesis and by RNase protection. Two protected regions were identified in the 
      3'UTR of tPA mRNA in primary oocytes: the adenylation control element (ACE) and 
      the AAUAAA polyadenylation signal. These sequences were previously shown to be 
      involved in the translational control of injected reporter transcripts. During 
      the first hour of meiotic maturation, part of the ACE and the AAUAAA 
      hexanucleotide became accessible to hybridization, suggesting a partial unmasking 
      of the 3'UTR of this mRNA before it becomes translationally competent. Our 
      results demonstrate that in vivo antisense oligodeoxynucleotide mapping can 
      reveal the dynamics of regulatory features of a native mRNA in the context of the 
      intact cell. They suggest that specific regions in the 3'UTR of tPA mRNA function 
      as cis-acting masking determinants involved in the silencing of tPA mRNA in 
      primary oocytes.
FAU - Stutz, A
AU  - Stutz A
AD  - Department of Morphology, University of Geneva Medical School, Switzerland. 
      Andre.stutz@medecine.unige.ch
FAU - Huarte, J
AU  - Huarte J
FAU - Gubler, P
AU  - Gubler P
FAU - Conne, B
AU  - Conne B
FAU - Belin, D
AU  - Belin D
FAU - Vassalli, J D
AU  - Vassalli JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Meiosis/drug effects/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oligonucleotides, Antisense/*genetics/pharmacology
MH  - Oocytes/drug effects/growth & development/*metabolism
MH  - Protein Biosynthesis/drug effects
MH  - RNA, Messenger/*genetics/metabolism
MH  - Tissue Plasminogen Activator/biosynthesis/genetics
PMC - PMC232022
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
PMCR- 1997/04/01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PHST- 1997/04/01 00:00 [pmc-release]
AID - 10.1128/MCB.17.4.1759 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1997 Apr;17(4):1759-67. doi: 10.1128/MCB.17.4.1759.