PMID- 9122226
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 6
DP  - 1997 Mar 18
TI  - Pluripotent hemopoietic stem cells are c-kit<low.
PG  - 2513-7
AB  - Pluripotent hemopoietic stem cells (P-HSCs) were thought to be c-kit+, but recent 
      reports indicate that they are c-kit(low). In the present report, we provide 
      evidence using Ly5 congenic mice that P-HSCs are c-kit(<low). Lineage-negative 
      (Lin-)/CD71- cells among bone marrow cells (BMCs) from C57BL/6 Ly5.1 mice were 
      separated into major histocompatibility complex class I(high) (class 
      I(high))/c-kit(low) and class I(high)/ c-kit(<low) populations. Each population 
      (500 cells) was transplanted into lethally (9.0 Gy) irradiated C57BL/6 Ly5.2 
      congenic mice along with Ly5.2 (2 x 10(5)) compromised cells. Donor-derived 
      Ly5.1+ cells were detected 6 months after transplantation in primary recipients 
      reconstituted with either class I(high)/c-kit(low) or class I(high)/c-kit(<low) 
      cells. BMCs (1 x 10(6)) from the primary recipients were further transplanted 
      into secondary recipients (Ly5.2 mice) to assess their long term repopulating 
      activity. Six months after bone marrow transplantation, Ly5.1+ cells in all 
      lineages were detected only in secondary recipients that had been given BMCs from 
      the primary recipients reconstituted with class I(high)/c-kit(<low) cells but not 
      in cells that were class I(high)/c-kit(low). When the BMCs (1 x 10(6)) of these 
      secondary recipients were further transplanted into tertiary recipients, all 
      tertiary recipients that had been given BMCs from the secondary recipients 
      originally reconstituted with Lin-/CD71-/class I(high)/c-kit(low) cells died 
      within 10 days whereas all six tertiary recipients originally reconstituted with 
      Lin-/CD71-/class I(high)/c-kit(<low) cells showed donor (Ly5.1+)-derived cells in 
      their peripheral blood. In the single tertiary recipient that was killed, 
      donor-derived T cells, B cells, macrophages, and granulocytes also were detected 
      in several major hematolymphoid organs. The remaining five mice continue to 
      survive more than 6 months after the tertiary bone marrow transplantation.
FAU - Doi, H
AU  - Doi H
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Inaba, M
AU  - Inaba M
FAU - Yamamoto, Y
AU  - Yamamoto Y
FAU - Taketani, S
AU  - Taketani S
FAU - Mori, S I
AU  - Mori SI
FAU - Sugihara, A
AU  - Sugihara A
FAU - Ogata, H
AU  - Ogata H
FAU - Toki, J
AU  - Toki J
FAU - Hisha, H
AU  - Hisha H
FAU - Inaba, K
AU  - Inaba K
FAU - Sogo, S
AU  - Sogo S
FAU - Adachi, M
AU  - Adachi M
FAU - Matsuda, T
AU  - Matsuda T
FAU - Good, R A
AU  - Good RA
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
GR  - 5628-12/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-kit/analysis/*biosynthesis/genetics
MH  - Spleen/cytology
MH  - Thymus Gland/cytology
PMC - PMC20119
EDAT- 1997/03/18 00:00
MHDA- 1997/03/18 00:01
PMCR- 1997/09/18
CRDT- 1997/03/18 00:00
PHST- 1997/03/18 00:00 [pubmed]
PHST- 1997/03/18 00:01 [medline]
PHST- 1997/03/18 00:00 [entrez]
PHST- 1997/09/18 00:00 [pmc-release]
AID - 3886 [pii]
AID - 10.1073/pnas.94.6.2513 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2513-7. doi: 10.1073/pnas.94.6.2513.