PMID- 9124388
OWN - NLM
STAT- MEDLINE
DCOM- 19970424
LR  - 20180109
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 272
IP  - 2 Pt 1
DP  - 1997 Feb
TI  - Nitrofen dose-dependent gestational day-specific murine lung hypoplasia and 
      left-sided diaphragmatic hernia.
PG  - L362-71
AB  - 2,4-Dichlorophenyl-p-nitrophenyl ether (nitrofen) is known to induce pulmonary 
      hypoplasia (PH) with or without diaphragmatic hernias (DH) in rats and mice. We 
      determined the timing of administration and dose of nitrofen needed to create 
      left-sided DH and PH in fetal mice. Time-dated pregnant CD-1 mice were gavaged 
      with various doses of nitrofen in the later one-half of gestational days (GD) 
      8-11. Fetuses were removed by laparotomy at GD 14, fixed, and evaluated 
      histologically. Fetal lung size was inversely related to nitrofen dose. 
      Morphometric analysis of normal and nitrofen-exposed hypoplastic lungs at the 
      pseudoglandular stage revealed significant differences in lung length, surface 
      area, and in the number of airways. Left-sided DH were observed in a 
      "dorsolateral" position accompanied by PH in approximately 30% of GD 14 fetuses 
      exposed to 25 mg nitrofen on GD 8. A minimal portion of liver was present in the 
      hernia. The lungs of fetuses exposed on GD 9, 10, and 11 progressed to near 
      normal size. Murine fetuses exposed to 25 mg nitrofen on GD 8 resulted in PH and 
      DH, whereas other doses created dose-dependent PH alone or none at all on GD 11. 
      Our study established that, to create left-sided DH and PH in murine fetuses, 
      nitrofen dose specificity and time of administration during gestation were 
      crucial.
FAU - Cilley, R E
AU  - Cilley RE
AD  - Department of Surgery, The Pennsylvania State University College of Medicine, 
      Milton S. Hershey Medical Center, Hershey 17033, USA.
FAU - Zgleszewski, S E
AU  - Zgleszewski SE
FAU - Krummel, T M
AU  - Krummel TM
FAU - Chinoy, M R
AU  - Chinoy MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Phenyl Ethers)
RN  - N71UYG034A (nitrofen)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Fetus/anatomy & histology
MH  - Gestational Age
MH  - Hernia, Diaphragmatic/*chemically induced/pathology
MH  - Lung/*abnormalities/*drug effects/embryology
MH  - Mice/embryology
MH  - Mice, Inbred Strains
MH  - Microscopy, Electron
MH  - Phenyl Ethers/*pharmacology
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1152/ajplung.1997.272.2.L362 [doi]
PST - ppublish
SO  - Am J Physiol. 1997 Feb;272(2 Pt 1):L362-71. doi: 10.1152/ajplung.1997.272.2.L362.