PMID- 9125730 OWN - NLM STAT- MEDLINE DCOM- 19970602 LR - 20091119 IS - 0006-8969 (Print) IS - 0006-8969 (Linking) VI - 49 IP - 3 DP - 1997 Mar TI - [Expression of HGF and c-met/HGF receptor mRNA in brains of normal rats and in phaeochromocytoma cells PC-12 of rats treated with NGF]. PG - 252-9 AB - Gene expression of HGF and c-met proto-oncogene was examined during rat brain development and in cultured PC-12 cells, using reverse-transcriptase (RT)-polymerase chain reaction (PCR) technique. The both mRNAs of HGF and c-met proto-oncogene were remained at low levels in the middle and late stages of gestation (E-13 and E-18). After birth, and the level of both mRNA expression suddenly increased. During P-1 and P-12, their high level of expression continued and then decreased in P-20 and adult brain. Both HGF mRNA and c-met photo-oncogene mRNA were transiently expressed between day 2 and day 5, and disappeared in cultured PC-12 cells treated with NGF. The neurites of PC-12 cells that were treated with anti-sense oligonucleotides of HGF and c-met proto-oncogene, were shorter and fewer in number than untreated control cells. We conclude that neurite extension of PC-12 cells treated with NGF may ensue by way of c-MET protein activation and signal transduction pathways. Thus, c-MET protein activation and up-regulation of the two mRNAs may also play an important role in neuronal maturation in the developing rat brain. FAU - Ukita, H AU - Ukita H AD - Neuropathology Research Group, Hokuto Hospital, Hokkaido, Japan. FAU - Sawa, H AU - Sawa H FAU - Murakami, H AU - Murakami H FAU - Satone, A AU - Satone A FAU - Kamada, H AU - Kamada H FAU - Saito, I AU - Saito I LA - jpn PT - English Abstract PT - Journal Article PL - Japan TA - No To Shinkei JT - No to shinkei = Brain and nerve JID - 0413550 RN - 0 (Nerve Growth Factors) RN - 0 (Oligonucleotides, Antisense) RN - 0 (RNA, Messenger) RN - 67256-21-7 (Hepatocyte Growth Factor) RN - EC 2.7.10.1 (Proto-Oncogene Proteins c-met) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) SB - IM MH - Animals MH - Brain/growth & development/*metabolism/pathology MH - Hepatocyte Growth Factor/*genetics MH - Nerve Growth Factors/*pharmacology MH - Oligonucleotides, Antisense/pharmacology MH - Pheochromocytoma/genetics/*metabolism/pathology MH - Polymerase Chain Reaction MH - Proto-Oncogene Proteins c-met MH - RNA, Messenger/*metabolism MH - Rats MH - Receptor Protein-Tyrosine Kinases/*genetics MH - Tumor Cells, Cultured/drug effects EDAT- 1997/03/01 00:00 MHDA- 1997/03/01 00:01 CRDT- 1997/03/01 00:00 PHST- 1997/03/01 00:00 [pubmed] PHST- 1997/03/01 00:01 [medline] PHST- 1997/03/01 00:00 [entrez] PST - ppublish SO - No To Shinkei. 1997 Mar;49(3):252-9.