PMID- 9126820
OWN - NLM
STAT- MEDLINE
DCOM- 19970502
LR  - 20081121
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 46
IP  - 3
DP  - 1997 Mar
TI  - Pyrolysis mass spectrometry in epidemiological and population genetic studies of 
      Haemophilus influenzae.
PG  - 204-7
AB  - Haemophilus influenzae serotype b (Hib) vaccines have reduced the amount of 
      invasive Hib disease in immunised infants. However, Hib disease remains in 
      unvaccinated infants and adults and non-capsulate H. influenzae (NCHi) still 
      causes infections, including outbreaks of respiratory disease. Characterisation 
      of strains and the bacterial population as a whole is therefore necessary to 
      detect outbreaks of infection with NCHi or changes in the population, for 
      example, to vaccine-resistant clones of Hib. The rapid, simple and objective 
      technique of pyrolysis mass spectrometry (PMS) was investigated as an alternative 
      to current complex, subjective methods. PMS was compared with ribotyping and 
      multilocus enzyme electrophoresis (MLEE) for population genetic analyses of Hib 
      and with ribotyping and protein profiling for epidemiological analyses of NCHi. 
      PMS clustered all the isolates of Hib together whereas MLEE and ribotyping 
      distinguished certain clones - this is probably because the three methods examine 
      different (and unrelated) characteristics of the organisms. The PMS results were 
      essentially similar to those from ribotyping and protein profiling for the 
      epidemiological analyses of outbreaks of NCHi disease. Therefore, PMS is probably 
      unsuitable for comparisons of Hib populations but it is a useful addition to the 
      arsenal of techniques for the characterisation of NCHi.
FAU - Leaves, N I
AU  - Leaves NI
AD  - Oxford Public Health Laboratory, John Radcliffe Hospital, Headington, Oxford.
FAU - Sisson, P R
AU  - Sisson PR
FAU - Freeman, R
AU  - Freeman R
FAU - Jordens, J Z
AU  - Jordens JZ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Adult
MH  - Bacterial Proteins/analysis
MH  - *Bacterial Typing Techniques
MH  - Cluster Analysis
MH  - Cross Infection/epidemiology/*microbiology
MH  - Disease Outbreaks
MH  - Genetics, Population
MH  - Haemophilus Infections/epidemiology/*microbiology
MH  - Haemophilus influenzae/*classification/genetics
MH  - Hot Temperature
MH  - Humans
MH  - Infant
MH  - Mass Spectrometry/*methods
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1099/00222615-46-3-204 [doi]
PST - ppublish
SO  - J Med Microbiol. 1997 Mar;46(3):204-7. doi: 10.1099/00222615-46-3-204.