PMID- 9128288
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20100825
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 41
IP  - 5
DP  - 1997 May
TI  - Expression of hepatocyte growth factor/scatter factor and its receptor, MET, 
      suggests roles in human embryonic organogenesis.
PG  - 657-65
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is secreted by mesenchymal cells 
      and elicits proliferation, motility, differentiation, and morphogenesis of 
      epithelia and other cells. These effects are mediated by binding to MET, a 
      receptor tyrosine kinase. Genetically engineered mice lacking HGF/SF die in utero 
      due to a failure of placental and hepatocyte differentiation, but little 
      information exists regarding the expression of this signaling system in human 
      development. Using reverse transcriptase-polymerase chain reaction, Western 
      blots, and immunohistochemistry, we report that HGF/SF and MET are expressed 
      during critical early periods of human organogenesis from 6 to 13 wk of 
      gestation. Organs that expressed both genes included liver, metanephric kidney, 
      intestine, and lung, each of which develop by inductive interactions between 
      mesenchyme and epithelia. Of all organs studied, the placenta contained the 
      highest levels of HGF/SF protein, and MET was detected in trophoblastic cells of 
      chorionic villi as early as the 5th wk of gestation. Finally, examination of a 
      human multicystic dysplastic kidney demonstrated that malformed, 
      hyperproliferative tubules expressed MET, whereas HGF/SF protein was 
      immunolocalized to the same epithelia and also to the surrounding 
      undifferentiated cells. Hence HGF/SF might be an important growth factor in 
      normal human embryogenesis and may additionally play a role in human organ 
      malformations.
FAU - Kolatsi-Joannou, M
AU  - Kolatsi-Joannou M
AD  - Developmental Biology, Institute of Child Health, London, United Kingdom.
FAU - Moore, R
AU  - Moore R
FAU - Winyard, P J
AU  - Winyard PJ
FAU - Woolf, A S
AU  - Woolf AS
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Embryo, Mammalian
MH  - *Embryonic and Fetal Development
MH  - Fetus
MH  - *Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - Hepatocyte Growth Factor/*biosynthesis
MH  - Humans
MH  - Intestines/embryology
MH  - Kidney/embryology
MH  - Liver/embryology
MH  - Lung/embryology
MH  - Mice
MH  - Mice, Transgenic
MH  - Organ Specificity
MH  - Polycystic Kidney Diseases/embryology/pathology
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-met
MH  - RNA, Messenger/biosynthesis
MH  - Receptor Protein-Tyrosine Kinases/*biosynthesis
MH  - Transcription, Genetic
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1203/00006450-199705000-00010 [doi]
PST - ppublish
SO  - Pediatr Res. 1997 May;41(5):657-65. doi: 10.1203/00006450-199705000-00010.