PMID- 9128858
OWN - NLM
STAT- MEDLINE
DCOM- 19970623
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 65
IP  - 1
DP  - 1997 Apr
TI  - Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection.
PG  - 19-25
AB  - Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, 
      integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in 
      two model cell lines for chronic HIV infection. In the promonocytic cell line 
      U1/HIV-1, the two integrated copies of proviral HIV-DNA were located on the short 
      arm of one copy of chromosome X (band Xp21) and on a rearranged chromosome 6. In 
      the T-cell line 8E5/LAV, the single copy of proviral HIV-DNA was integrated into 
      chromosomal bands 13q14-q21, which are known to contain common fragile sites. In 
      addition, ultra-sensitive FISH was used for the detection of HIV provirus 
      sequences in interphase cells, achieving a high hybridization efficiency. This 
      application allows the analysis of the HIV status on a single cell level and thus 
      may become a useful tool for the study of HIV infection.
FAU - Deichmann, M
AU  - Deichmann M
AD  - Klinische Kooperationseinheit Molekulare Hamatologie/Onkologie, Deutsches 
      Krebsforschungszentrum, Heidelberg, Germany.
FAU - Bentz, M
AU  - Bentz M
FAU - Haas, R
AU  - Haas R
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 6
MH  - Genome, Viral
MH  - HIV Infections/*genetics/physiopathology
MH  - HIV-1/*genetics/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - X Chromosome
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0166093496021647 [pii]
AID - 10.1016/s0166-0934(96)02164-7 [doi]
PST - ppublish
SO  - J Virol Methods. 1997 Apr;65(1):19-25. doi: 10.1016/s0166-0934(96)02164-7.