PMID- 9129803
OWN - NLM
STAT- MEDLINE
DCOM- 19970630
LR  - 20181113
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 72
IP  - 5
DP  - 1997 May
TI  - Control of actin moving trajectory by patterned poly(methylmethacrylate) tracks.
PG  - 1997-2001
AB  - Poly(methylmethacrylate) (PMMA), a photoresist polymer, was found to be useful 
      for immobilizing heavy meromyosin (HMM) molecules while retaining their abilities 
      to support the movement of actin filaments. PMMA substrate was spin-coated on a 
      coverslip, and various shapes of PMMA tracks, such as straight lines, concentric 
      circles, and alphabetical letters, were fabricated by UV photolithography. An 
      observation by a Tapping mode atomic force microscope (AFM) shows that the 
      typical circular tracks were 1-2 microns wide and about 200 nm high. In in vitro 
      motility assay, a solution of HMM molecules was applied to immobilize the 
      molecules on the tracks by adsorption, and movement of actin filaments labeled 
      with tetramethylrhodamine-phalloidin were observed in the presence of ATP by 
      using an epifluorescence microscope and an image-intensified CCD camera. Actin 
      filaments were seen to move precisely only on the PMMA tracks, and their traces 
      drew the exact shapes of the tracks. The mean velocity of actin movement on the 
      PMMA was 4.5 mm/s at 25 degrees C, and it was comparable to that on a 
      conventionally used nitrocellulose film.
FAU - Suzuki, H
AU  - Suzuki H
AD  - Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan. 
      suzuki@crl.go.jp
FAU - Yamada, A
AU  - Yamada A
FAU - Oiwa, K
AU  - Oiwa K
FAU - Nakayama, H
AU  - Nakayama H
FAU - Mashiko, S
AU  - Mashiko S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Actins)
RN  - 0 (Methylmethacrylates)
RN  - 0 (Polymers)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Actins/*chemistry
MH  - Animals
MH  - Biophysical Phenomena
MH  - Biophysics
MH  - Methylmethacrylates/chemistry
MH  - Microscopy, Atomic Force/methods
MH  - Microscopy, Fluorescence/methods
MH  - Myosins/chemistry
MH  - Polymers/chemistry
MH  - Rabbits
PMC - PMC1184395
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
PMCR- 1998/05/01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
PHST- 1998/05/01 00:00 [pmc-release]
AID - S0006-3495(97)78844-1 [pii]
AID - 10.1016/S0006-3495(97)78844-1 [doi]
PST - ppublish
SO  - Biophys J. 1997 May;72(5):1997-2001. doi: 10.1016/S0006-3495(97)78844-1.