PMID- 9130587 OWN - NLM STAT- MEDLINE DCOM- 19970519 LR - 20190610 IS - 0006-3002 (Print) IS - 0006-3002 (Linking) VI - 1351 IP - 3 DP - 1997 Apr 10 TI - Specific inhibition of in vitro reverse transcription using antisense oligonucleotides targeted to the TAR regions of HIV-1 and HIV-2. PG - 249-55 AB - Antisense oligonucleotides (ODNs) overlapping the stem-loop structure of the trans-activating responsive (TAR) element at the 5' end of HIV-1 and HIV-2 viral RNAs were tested for their inhibitory effect on cDNA synthesis by HIV-1 and HIV-2 reverse transcriptases (RT). Inhibition of reverse transcription is sequence-specific and enhanced by the presence of the RT-associated RNase H activity. The degree of inhibition obtained with the anti-TAR antisense is significantly higher than with other HIV-1 targeted antisense ODNs used before [1]. Gel retardation showed a stable specific complex between the 16- and 25-mer anti-TAR HIV-1 selected ODNs and the target region. No complex was observed with a non-inhibitor 22-mer anti-TAR ODN and with the corresponding control sequences. Targeting of the first stem-loop in the 5' region of HIV-2 RNA by anti-TAR ODNs inhibited very strongly reverse transcription by HIV-2 RT. The structure of the antisense and the target sequence affect annealing efficiency and hence the degree of inhibition of reverse transcription. FAU - Boulme, F AU - Boulme F AD - Laboratoire Reger, Institut de Biochimie et Genetique Cellulaires du CNRS, IBGC-CNRS, Bordeaux, France. FAU - Perala-Heape, M AU - Perala-Heape M FAU - Sarih-Cottin, L AU - Sarih-Cottin L FAU - Litvak, S AU - Litvak S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Biochim Biophys Acta JT - Biochimica et biophysica acta JID - 0217513 RN - 0 (DNA, Complementary) RN - 0 (DNA, Viral) RN - 0 (Nucleic Acid Heteroduplexes) RN - 0 (Oligonucleotides, Antisense) RN - 0 (RNA, Viral) RN - 0 (Reverse Transcriptase Inhibitors) RN - EC 2.7.7.- (reverse transcriptase, Human immunodeficiency virus 2) RN - EC 2.7.7.49 (HIV Reverse Transcriptase) RN - EC 2.7.7.49 (RNA-Directed DNA Polymerase) RN - EC 3.1.26.4 (Ribonuclease H) SB - IM MH - Base Sequence MH - DNA, Complementary/biosynthesis/drug effects MH - DNA, Viral/biosynthesis/drug effects MH - Electrophoresis/methods MH - HIV Long Terminal Repeat/*drug effects/genetics MH - HIV Reverse Transcriptase MH - HIV-1/drug effects/*genetics MH - Molecular Sequence Data MH - Nucleic Acid Heteroduplexes MH - Oligonucleotides, Antisense/chemistry/*genetics/*pharmacology MH - RNA, Viral/chemistry/metabolism MH - RNA-Directed DNA Polymerase/drug effects/*genetics MH - Reverse Transcriptase Inhibitors/chemistry/pharmacology MH - Ribonuclease H/drug effects/genetics/metabolism MH - Virus Replication/drug effects/genetics EDAT- 1997/04/10 00:00 MHDA- 1997/04/10 00:01 CRDT- 1997/04/10 00:00 PHST- 1997/04/10 00:00 [pubmed] PHST- 1997/04/10 00:01 [medline] PHST- 1997/04/10 00:00 [entrez] AID - S0167-4781(97)00026-2 [pii] AID - 10.1016/s0167-4781(97)00026-2 [doi] PST - ppublish SO - Biochim Biophys Acta. 1997 Apr 10;1351(3):249-55. doi: 10.1016/s0167-4781(97)00026-2.