PMID- 9131053
OWN - NLM
STAT- MEDLINE
DCOM- 19970522
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1319
IP  - 2-3
DP  - 1997 Apr 11
TI  - Source of ATP for hexokinase-catalyzed glucose phosphorylation in tumor cells: 
      dependence on the rate of oxidative phosphorylation relative to that of 
      extramitochondrial ATP generation.
PG  - 319-30
AB  - We isolated highly intact and tightly coupled mitochondria from the rat ascites 
      hepatoma cell line AH130 by disruption of the cell membrane by nitrogen 
      cavitation. These isolated mitochondria were found to have essentially the same 
      functional properties as rat liver mitochondria, but unlike the latter, 
      hexokinase (HK) was bound to their membrane. Using the tumor mitochondrial 
      preparation, we examined the source of ATP for phosphorylation of glucose by HK 
      under conditions in which intra- and extramitochondrial ATP-generation systems 
      operated separately or together. Results showed that the membrane-bound HK 
      utilized ATP derived from the most efficiently operating ATP generation system, 
      i.e., oxidative phosphorylation. However, when the rate of extramitochondrial ATP 
      generation was much greater than that of oxidative phosphorylation, HK used ATP 
      from the extramitochondrial ATP-generation system.
FAU - Shinohara, Y
AU  - Shinohara Y
AD  - Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
FAU - Sagawa, I
AU  - Sagawa I
FAU - Ichihara, J
AU  - Ichihara J
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Terao, K
AU  - Terao K
FAU - Terada, H
AU  - Terada H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenosine Diphosphate/metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Cell Fractionation
MH  - Glucose/*metabolism
MH  - Hexokinase/*metabolism
MH  - Kinetics
MH  - Liver Neoplasms, Experimental/*metabolism
MH  - Microscopy, Electron
MH  - Mitochondria/*metabolism/ultrastructure
MH  - Mitochondria, Liver/*metabolism/ultrastructure
MH  - Models, Biological
MH  - *Oxidative Phosphorylation
MH  - Oxygen Consumption
MH  - Phosphorylation
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1997/04/11 00:00
MHDA- 1997/04/11 00:01
CRDT- 1997/04/11 00:00
PHST- 1997/04/11 00:00 [pubmed]
PHST- 1997/04/11 00:01 [medline]
PHST- 1997/04/11 00:00 [entrez]
AID - S0005-2728(97)00002-9 [pii]
AID - 10.1016/s0005-2728(97)00002-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1997 Apr 11;1319(2-3):319-30. doi: 
      10.1016/s0005-2728(97)00002-9.