PMID- 9132652
OWN - NLM
STAT- MEDLINE
DCOM- 19970501
LR  - 20190512
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 12
IP  - 2
DP  - 1997 Feb
TI  - Inverse MCP-1/IL-8 ratio in effluents of CAPD patients with peritonitis and in 
      isolated cultured human peritoneal macrophages.
PG  - 315-20
AB  - An important event in intraperitoneal inflammation is the influx of leukocytes 
      into the peritoneal cavity. Chemokines such as interleukin-8 (IL-8) and monocyte 
      chemoattractant protein-1 (MCP-1) play a major role in the recruitment of immune 
      cells to the site of inflammation. We determined the concentrations of two 
      members of the chemokine family, IL-8 and MCP-1, in the dialysate effluents of 18 
      continuous ambulatory peritoneal dialysis (CAPD) patients with peritonitis and of 
      18 non-infected CAPD patients by specific enzyme-linked immunosorbent assays 
      (ELISA). Isolated peritoneal macrophages (PMs) from CAPD peritonitis patients 
      were cultured and IL-8 and MCP-1 production was determined on protein (ELISA) and 
      mRNA level (Northern blot) at designated timepoints over a 72-h culture period. 
      PMs from non-infected patients served as controls. Much higher concentrations of 
      IL-8 and MCP-1 were found in dialysate effluents of peritonitis patients than in 
      effluents of non-infected patients: IL-8 2.39 +/- 1.15 vs 0.05 +/- 0.01 ng/ml and 
      MCP-1 22.5 +/- 6.27 vs 0.42 +/- 0.07 ng/ml. IL-8 and MCP-1 release by cultured 
      PMs from peritonitis patients and non-infected patients revealed significant 
      differences: IL-8 40.3 +/- 2.2 ng/ml after 3 h and 194.2 +/- 34.9 ng/ml after 12 
      h compared to 21.02 +/- 6.15 ng/ml after 3 h and 89.64 +/- 30.28 ng/ml after 12 
      h, respectively; MCP-1 3.3 +/- 0.9 ng/ml after 3 h and 25.7 +/- 7.4 ng/ml after 
      12 h compared to 1.1 +/- 0.2 ng/ml and 1.8 +/- 0.2 ng/ml, respectively. 
      Interestingly, the ratio of IL-8 to MCP-1 concentrations in the dialysate 
      effluents (1:9.4) is reversed in the supernatants of cultured PMs. In the 
      effluents and in the culture supernatants of PMs from CAPD peritonitis patients 
      high amounts of IL-8 and MCP-1 are detectable, suggesting that PMs are an 
      important source for these chemokines during peritonitis. Because of the inverse 
      ratio of IL-8 and MCP-1 in the effluents and culture supernatants it can be 
      assumed that PMs are responsible for the MCP-1 concentration to a lesser extent 
      than for the IL-8 concentration in the effluents.
FAU - Sach, M
AU  - Sach M
AD  - Department of Nephrology, University Hospital Freiburg, Germany.
FAU - Bauermeister, K
AU  - Bauermeister K
FAU - Burger, J A
AU  - Burger JA
FAU - Loetscher, P
AU  - Loetscher P
FAU - Elsner, J
AU  - Elsner J
FAU - Schollmeyer, P
AU  - Schollmeyer P
FAU - Dobos, G J
AU  - Dobos GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*analysis/biosynthesis/genetics
MH  - Humans
MH  - Interleukin-8/*analysis/biosynthesis/genetics
MH  - Macrophages, Peritoneal/*metabolism
MH  - *Peritoneal Dialysis, Continuous Ambulatory
MH  - Peritonitis/*metabolism
MH  - RNA, Messenger/analysis
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1093/ndt/12.2.315 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 1997 Feb;12(2):315-20. doi: 10.1093/ndt/12.2.315.