PMID- 9134567
OWN - NLM
STAT- MEDLINE
DCOM- 19970702
LR  - 20190909
IS  - 0932-0113 (Print)
IS  - 0932-0113 (Linking)
VI  - 83
IP  - 4
DP  - 1997
TI  - In vitro Entamoeba histolytica adhesion to human endothelium: a comparison using 
      two strains of different virulence.
PG  - 397-400
AB  - Extraintestinal dissemination of Entamoeba histolytica is frequently manifested 
      by the life-threatening amebic liver abscess (ALA). The hepatic establishment of 
      amebas implies invasion of blood vessels and contact with the endothelium. By 
      means of a fluorescence-based quantitative adhesion assay, we assessed the 
      binding to human endothelial cells of two E. histolytica strains of different 
      virulence. The highly virulent strain (L-A) adhered substantially more strongly 
      to unstimulated endothelium than the non-virulent one (BG3). Attachment of L-A 
      was increased by treatment of endothelial cells with interleukin-1 beta (IL1 
      beta). Other proinflammatory cytokines such as interferon-gamma (IFN gamma) and 
      tumor necrosis factor-alpha (TNF alpha) did not modify the spontaneous adhesion 
      capacity of amebas. For purposes of comparison we also performed adhesion of the 
      parasites to skin fibroblasts. Adhesion to this cell type was quite low (< 10%). 
      Parasite virulence, differential adhesive capacity to endothelial cells, and 
      modulation of the latter phenomenon by proinflammatory factors (IL1 beta) may 
      influence the evolution and outcome of extraintestinal amebiasis, especially 
      hepatic abscesses.
FAU - Flores-Romo, L
AU  - Flores-Romo L
AD  - Glaxo Institute for Molecular Biology, Geneva, Switzerland.
FAU - Estrada-Garcia, T
AU  - Estrada-Garcia T
FAU - Shibayama-Salas, M
AU  - Shibayama-Salas M
FAU - Campos-Rodriguez, R
AU  - Campos-Rodriguez R
FAU - Bacon, K
AU  - Bacon K
FAU - Martinez-Palomo, A
AU  - Martinez-Palomo A
FAU - Tsutsumi, V
AU  - Tsutsumi V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cytokines/pharmacology
MH  - Endothelium, Vascular/*parasitology
MH  - Entamoeba histolytica/*pathogenicity/*physiology
MH  - Fibroblasts/cytology/physiology
MH  - Genetic Variation
MH  - Humans
MH  - Skin/cytology
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s004360050271 [doi]
PST - ppublish
SO  - Parasitol Res. 1997;83(4):397-400. doi: 10.1007/s004360050271.