PMID- 9136947
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 97
IP  - 1
DP  - 1997 Apr
TI  - Which compartments are involved in Philadelphia-chromosome positive chronic 
      myeloid leukaemia? An answer at the single cell level by combining 
      May-Grunwald-Giemsa staining and fluorescence in situ hybridization techniques.
PG  - 99-106
AB  - Chronic myeloid leukaemia (CML) is believed to represent a stem cell disorder 
      involving all three cell lineages. The typical chromosomal aberration, the 
      Philadelphia chromosome, is the translocation (9;22)(q34;q11). Several studies 
      with cytogenetics, fluorescence in situ hybridization (FISH), or polymerase chain 
      reaction have investigated the presence of the t(9;22) in different cell 
      compartments. However, questions still remain. In six cases of CML we combined 
      the standard May-Grunwald-Giemsa staining with FISH at the single-cell level and 
      were able to demonstrate that not only all maturation stages of granulopoiesis, 
      erythropoiesis, and megakaryocytes, but also plasma cells, eosinophils, basophils 
      and monocytes carried the Philadelphia chromosome in 53-98% of samples. Using 
      immunological identification of single cells we were able to demonstrate that the 
      t(9;22) is detectable in 34% of CD3-positive T lymphocytes, in 32% of 
      CD19-positive B lymphocytes, and in 82% of CD34-positive precursor cells. The 
      results give new insight into the biology of CML and may have implications for 
      future therapeutic strategies.
FAU - Haferlach, T
AU  - Haferlach T
AD  - Department of Haematology and Oncology, University of Gottingen, Germany.
FAU - Winkemann, M
AU  - Winkemann M
FAU - Nickenig, C
AU  - Nickenig C
FAU - Meeder, M
AU  - Meeder M
FAU - Ramm-Petersen, L
AU  - Ramm-Petersen L
FAU - Schoch, R
AU  - Schoch R
FAU - Nickelsen, M
AU  - Nickelsen M
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Schoch, C
AU  - Schoch C
FAU - Gassmann, W
AU  - Gassmann W
FAU - Loffler, H
AU  - Loffler H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Azure Stains)
SB  - IM
CIN - Br J Haematol. 1997 Dec;99(3):707-8. PMID: 9401090
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Azure Stains
MH  - B-Lymphocytes/pathology
MH  - Cell Lineage
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Staining and Labeling
MH  - T-Lymphocytes/pathology
MH  - *Translocation, Genetic
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1997.9662656.x [doi]
PST - ppublish
SO  - Br J Haematol. 1997 Apr;97(1):99-106. doi: 10.1046/j.1365-2141.1997.9662656.x.