PMID- 9136970
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 97
IP  - 1
DP  - 1997 Apr
TI  - Anti-endothelial cell antibodies to the endothelial hybridoma cell line (EAhy926) 
      in systemic lupus erythematosus patients with antiphospholipid antibodies.
PG  - 227-32
AB  - The endothelial hybridoma (EAhy926) cell line was employed to clarify whether 
      antiphospholipid antibodies (aPA) [lupus anticoagulant (LA), antiprothrombin 
      antibody (aPT) and/or anticardiolipin antibody (aCL)] and anti-endothelial cell 
      antibodies (AECA) are identical, and establish whether beta2-glycoprotein I 
      (beta2-GPI) is needed for reactivity of aPA to endothelial cells. Ig-G AECA was 
      positive in 9/30 SLE patients with aPA (30.0%) and 10/22 SLE patients negative 
      for aPA (45.5%). Ig-M AECA was positive in one SLE patient with aPA and one SLE 
      patient without aPA. AECA-positivity was not significantly different among 
      unfixed, TNF-stimulated and fixed EAhy926. The influence of beta2-GPI on the 
      reactivity of serum to EAhy926 was minimal, and absorption experiments of serum 
      with cardiolipin-liposome/beta2-GPI or phosphatidylserine-liposome/prothrombin 
      gave little evidence of cross-reactivity of aPA and AECA. The results of our 
      study suggest that aPA and AECA may have partially cross-reacted, but were 
      different antibodies. However, further study is needed to clarify the 
      clinico-pathological significance of AECA.
FAU - Matsuda, J
AU  - Matsuda J
AD  - Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
FAU - Gotoh, M
AU  - Gotoh M
FAU - Gohchi, K
AU  - Gohchi K
FAU - Kawasugi, K
AU  - Kawasugi K
FAU - Tsukamoto, M
AU  - Tsukamoto M
FAU - Saitoh, N
AU  - Saitoh N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antibodies)
RN  - 0 (Antibodies, Anticardiolipin)
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (Autoantibodies)
RN  - 0 (Glycoproteins)
RN  - 0 (Lupus Coagulation Inhibitor)
RN  - 0 (anti-endothelial cell antibody)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 9001-26-7 (Prothrombin)
SB  - IM
MH  - Antibodies/*metabolism
MH  - Antibodies, Anticardiolipin/metabolism
MH  - Antibodies, Antiphospholipid/*metabolism
MH  - Autoantibodies/*metabolism
MH  - Cell Line
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Hybridomas/*immunology
MH  - Lupus Coagulation Inhibitor/metabolism
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Male
MH  - Prothrombin/*immunology
MH  - beta 2-Glycoprotein I
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1997.d01-2147.x [doi]
PST - ppublish
SO  - Br J Haematol. 1997 Apr;97(1):227-32. doi: 10.1046/j.1365-2141.1997.d01-2147.x.