PMID- 9139731
OWN - NLM
STAT- MEDLINE
DCOM- 19970616
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 19
DP  - 1997 May 9
TI  - Expression of small extracellular chondroitin/dermatan sulfate proteoglycans is 
      differentially regulated in human endothelial cells.
PG  - 12730-7
AB  - We have examined the expression of the small extracellular chondroitin/dermatan 
      sulfate proteoglycans (CS/DS PGs), biglycan, decorin, and PG-100, which is the 
      proteoglycan form of colony stimulating factor-1, in the human endothelial cell 
      line EA.hy 926. We have also examined whether modulation of the phenotype of 
      EA.hy 926 cells by tumor necrosis factor-alpha (TNF-alpha) is associated with 
      specific changes in the synthesis of these PGs. We demonstrate that EA.hy 926 
      cells, when they form monolayer cultures typical of macrovascular endothelial 
      cells, express and synthesize detectable amounts of biglycan and PG-100, but not 
      decorin. On SDS-polyacrylamide gel electrophoresis both PGs behave like proteins 
      of the relative molecular weight of approximately 250,000. TNF-alpha that changed 
      the morphology of the cells from a polygonal shape into a spindle shape and that 
      also stimulated the detachment of the cells from culture dish, markedly decreased 
      the net synthesis of biglycan, whereas the net synthesis of PG-100 was increased. 
      These changes were parallel with those observed at the mRNA level of the 
      corresponding PGs. The proportions of the different sulfated CS/DS disaccharide 
      units of PGs were not affected by TNF-alpha. Several other growth 
      factors/cytokines, such as interferon-gamma, fibroblast growth factors-2 (FGF-2) 
      and -7 (FGF-7), interleukin-1beta, and transforming growth factor-beta, unlike 
      TNF-alpha, modulated neither the morphology nor the biglycan expression of EA.hy 
      926 cells under the conditions used in the experiments. However, PG-100 
      expression was increased also in response to FGF-2 and -7 and transforming growth 
      factor-beta. None of the above cytokines, including TNF-alpha, was able to induce 
      decorin expression in the cells. Our results indicate that the regulatory 
      elements controlling the expression of the small extracellular CS/DS PGs in human 
      endothelial cells are different.
FAU - Nelimarkka, L
AU  - Nelimarkka L
AD  - Department of Medical Biochemistry, FIN-20520 Turku, Finland.
FAU - Kainulainen, V
AU  - Kainulainen V
FAU - Schonherr, E
AU  - Schonherr E
FAU - Moisander, S
AU  - Moisander S
FAU - Jortikka, M
AU  - Jortikka M
FAU - Lammi, M
AU  - Lammi M
FAU - Elenius, K
AU  - Elenius K
FAU - Jalkanen, M
AU  - Jalkanen M
FAU - Jarvelainen, H
AU  - Jarvelainen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BGN protein, human)
RN  - 0 (Biglycan)
RN  - 0 (Cytokines)
RN  - 0 (DCN protein, human)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 9007-28-7 (Chondroitin Sulfates)
SB  - IM
MH  - Biglycan
MH  - Blotting, Western
MH  - Cell Size/drug effects
MH  - Cells, Cultured
MH  - Chondroitin Sulfates/*metabolism
MH  - Cytokines/pharmacology
MH  - Decorin
MH  - Dermatan Sulfate/*metabolism
MH  - Endothelium/metabolism
MH  - Extracellular Matrix/metabolism
MH  - Extracellular Matrix Proteins
MH  - Humans
MH  - Macrophage Colony-Stimulating Factor/metabolism
MH  - Molecular Weight
MH  - Proteoglycans/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1997/05/09 00:00
MHDA- 1997/05/09 00:01
CRDT- 1997/05/09 00:00
PHST- 1997/05/09 00:00 [pubmed]
PHST- 1997/05/09 00:01 [medline]
PHST- 1997/05/09 00:00 [entrez]
AID - S0021-9258(18)40474-7 [pii]
AID - 10.1074/jbc.272.19.12730 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 May 9;272(19):12730-7. doi: 10.1074/jbc.272.19.12730.