PMID- 9139857
OWN - NLM
STAT- MEDLINE
DCOM- 19970523
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 71
IP  - 2
DP  - 1997 Apr 10
TI  - Expression and immunogenicity in rats of recombinant adenovirus 5 DNA plasmids 
      and vaccinia virus containing the HTLV-I env gene.
PG  - 300-7
AB  - The complete human T-cell leukemia virus type I (HTLV-I) env gene was inserted 
      into an expression cassette containing the adenovirus 5 major late promoter 
      (Ad5-MLP). Recombinant Ad5-HTLV-I-env was obtained by homologous recombination in 
      293 cells simultaneously transfected by the expression cassette and the genomic 
      DNA of Ad5. In vitro expression of the HTLV-I-env gene in the recombinant vector 
      was detected by immunofluorescence and Western blotting. Functional expression of 
      HTLV-I-env was confirmed by syncitium formation specifically in HeLa cells 
      infected with Ad5-HTLV-I-env. Two immunization regimens against HTLV-I were 
      tested in WKY and Fischer F-344 rats. The first involved WKY rats primed with 
      Ad5-HTLV-I-env or naked DNA plasmids containing the HTLV-I-env gene and boosted 
      with Ad5 containing the HTLV-I-env gp46 gene or with baculovirus-derived 
      recombinant gp46. No antibody against HTLV-I was detected, while HTLV-I-specific 
      cytotoxic T lymphocytes were recovered from all immunized groups but not from 
      controls. The second approach involved Fischer F-344 rats primed and boosted with 
      recombinant vaccinia virus containing the HTLV-I-env gene. Such rats developed 
      antibodies against the HTLV-I env gp21 and gp46 (non-neutralizing). After 
      challenge with human HTLV-I-producing cells (MT-2), both immunization regimens 
      were found to induce partial protection.
FAU - Kazanji, M
AU  - Kazanji M
AD  - Unite d'Epidemiologie des Virus Oncogenes, Institut Pasteur, Paris, France.
FAU - Bomford, R
AU  - Bomford R
FAU - Bessereau, J L
AU  - Bessereau JL
FAU - Schulz, T
AU  - Schulz T
FAU - de The, G
AU  - de The G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Blotting, Western
MH  - Cytotoxicity, Immunologic
MH  - DNA Primers/chemistry
MH  - DNA, Viral/*genetics
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Gene Expression Regulation, Viral/genetics/immunology
MH  - *Genes, env/genetics/immunology
MH  - HTLV-I Antibodies/analysis
MH  - HeLa Cells/virology
MH  - Human T-lymphotropic virus 1/*genetics/*immunology
MH  - Humans
MH  - Plasmids/genetics
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Inbred WKY
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Vaccinia virus/*genetics
MH  - Viral Envelope Proteins/immunology
EDAT- 1997/04/10 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/04/10 00:00
PHST- 1997/04/10 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/04/10 00:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19970410)71:2<300::AID-IJC27>3.0.CO;2-J [pii]
AID - 10.1002/(sici)1097-0215(19970410)71:2<300::aid-ijc27>3.0.co;2-j [doi]
PST - ppublish
SO  - Int J Cancer. 1997 Apr 10;71(2):300-7. doi: 
      10.1002/(sici)1097-0215(19970410)71:2<300::aid-ijc27>3.0.co;2-j.