PMID- 9141426
OWN - NLM
STAT- MEDLINE
DCOM- 19970528
LR  - 20171116
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 25
IP  - 5
DP  - 1997 May
TI  - Expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86) on human 
      hepatocellular carcinoma.
PG  - 1108-14
AB  - Costimulation mediated by costimulatory molecules, such as B7-1 and B7-2, which 
      are ligands for the CD28/cytolytic T lymphocyte associated antigen (CTLA)-4 
      counter-receptor, plays an important role in the induction of T cell-mediated 
      antitumor immunity. We investigated the expressions of B7-1, B7-2, and human 
      leukocyte antigen (HLA) class I in seven human hepatocellular carcinoma (HCC) 
      cell lines by reverse transcription-polymerase chain reaction (RT-PCR) and flow 
      cytometric analysis. RT-PCR showed that all these human HCC cell lines were 
      positive for B7-1 and B7-2 at the messenger RNA (mRNA) level. Flow cytometric 
      analysis revealed that they all expressed B7-1, B7-2, and HLA class I on the cell 
      surface. However, the expression levels of B7-1 and B7-2 were very low whereas 
      those of HLA class I were high. B7-1 and B7-2 expression could be increased by 
      treatment with interferon alpha and interferon gamma in a dose-dependent manner, 
      although the expression levels of B7-1 and B7-2 after interferon treatment 
      remained low. By transfecting Hep3B cells with a plasmid containing human B7-1 
      complementary cDNA (cDNA), we were able to establish Hep3B cell lines strongly 
      expressing B7-1. From mixed lymphocytes and tumor cultures analysis, the primary 
      cytolytic activity against parental Hep3B cells could be induced effectively by 
      B71-transfected Hep3B cells. These findings suggested that B7-1 gene transfer is 
      the best way to induce strong expression of this molecule and this might be 
      useful for immuno-gene therapy against human HCC.
FAU - Tatsumi, T
AU  - Tatsumi T
AD  - First Department of Medicine, Osaka University School of Medicine, Suita, Japan.
FAU - Takehara, T
AU  - Takehara T
FAU - Katayama, K
AU  - Katayama K
FAU - Mochizuki, K
AU  - Mochizuki K
FAU - Yamamoto, M
AU  - Yamamoto M
FAU - Kanto, T
AU  - Kanto T
FAU - Sasaki, Y
AU  - Sasaki Y
FAU - Kasahara, A
AU  - Kasahara A
FAU - Hayashi, N
AU  - Hayashi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Antigens, CD/*biosynthesis
MH  - B7-1 Antigen/*biosynthesis
MH  - B7-2 Antigen
MH  - Carcinoma, Hepatocellular/*immunology
MH  - *Cytotoxicity, Immunologic
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Liver Neoplasms/*immunology
MH  - Membrane Glycoproteins/*biosynthesis
MH  - Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S027091399700219X [pii]
AID - 10.1002/hep.510250511 [doi]
PST - ppublish
SO  - Hepatology. 1997 May;25(5):1108-14. doi: 10.1002/hep.510250511.