PMID- 9142127
OWN - NLM
STAT- MEDLINE
DCOM- 19970528
LR  - 20210102
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 3
IP  - 5
DP  - 1997 May
TI  - Induction of antitumor activity by immunization with fusions of dendritic and 
      carcinoma cells.
PG  - 558-61
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that prime naive 
      cytotoxic T-cells (CTLs). In this study, we have fused DCs with MC38 carcinoma 
      cells. The fusion cells were positive for major histocompatibility (MHC) class I 
      and II, costimulating molecules and intercellular cell adhesion molecule-1 
      (ICAM-1). The results show that the fusion cells stimulate naive T cells in the 
      primary mixed lymphocyte reaction (MLR) and induce MC38 tumor-specific CTLs in 
      vivo. Antibody-mediated depletion experiments demonstrate that induction of CD4+ 
      and CD8+ CTLs protects against challenge with tumor cells. We also show that 
      immunization with the fusion cells induces rejection of established metastases. 
      These findings represent the first demonstration that fusions of DCs and tumor 
      cells can be used in the treatment of cancer.
FAU - Gong, J
AU  - Gong J
AD  - Division of Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical 
      School, Boston, Massachusetts 02115, USA.
FAU - Chen, D
AU  - Chen D
FAU - Kashiwaba, M
AU  - Kashiwaba M
FAU - Kufe, D
AU  - Kufe D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (B7-1 Antigen)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adenocarcinoma/*immunology/pathology/prevention & control/therapy
MH  - Animals
MH  - Antigens, Neoplasm/analysis
MH  - B7-1 Antigen/analysis
MH  - Cell Fusion
MH  - Dendritic Cells/*immunology/transplantation
MH  - Immunotherapy, Adoptive/methods
MH  - Intercellular Adhesion Molecule-1/analysis
MH  - Lung Neoplasms/secondary/therapy
MH  - *Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Neoplasms, Experimental/immunology/pathology/prevention & control/*therapy
MH  - T-Lymphocytes/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1038/nm0597-558 [doi]
PST - ppublish
SO  - Nat Med. 1997 May;3(5):558-61. doi: 10.1038/nm0597-558.