PMID- 9143826
OWN - NLM
STAT- MEDLINE
DCOM- 19970715
LR  - 20190909
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 16
IP  - 4
DP  - 1997 Apr
TI  - Modified interphase cytogenetics technique as an adjunct in the analysis of 
      atypical cells in body fluids.
PG  - 331-5
AB  - Our objective was to determine the value of a modified interphase cytogenetics 
      technique (MICT) by fluorescence in situ hybridization (FISH) in the study of 
      atypical cells in body fluids in previously stained slides, allowing a direct 
      morphologic-cytogenetic correlation. Thirty-five cases (29 bladder washes, four 
      pleural fluids, two ascitic fluids) initially diagnosed as "atypical" with 
      subsequent histologic confirmation were included. Histologically, there were 25 
      malignant four dysplastic, and six benign lesions. Previously Papanicolaou or 
      Diff-Quick-stained slides were marked to determine the location of the cells of 
      interest prior to FISH analysis. A pretreatment modification using pepsin 
      digestion was utilized. Chromosome-specific probe 8 (Vysis) was used to detect 
      numerical chromosomal abnormalities (NCA) involving chromosome 8. Various NCA 
      (aneuploid) were detected in the atypical cells of histologically proven 
      malignant cases but not in the benign cases. Using histology as a "gold 
      standard," FISH has a sensitivity of 83% and a specificity of 100%. In conclusion 
      this study shows that a MICT by FISH on previously stained slides can serve as an 
      adjunct in the study of atypical cells in body fluids. This technique allows a 
      direct morphologic-cytogenetic correlation which in the future may aid in the 
      better understanding of carcinogenesis.
FAU - Cajulis, R S
AU  - Cajulis RS
AD  - Consolidated Medical Laboratories, Lake Forest Hospital, Illinois, USA.
FAU - Yu, G H
AU  - Yu GH
FAU - Gokaslan, S T
AU  - Gokaslan ST
FAU - Hidvegi, D F
AU  - Hidvegi DF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Body Fluids/cytology
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Sensitivity and Specificity
EDAT- 1997/04/01 00:00
MHDA- 2000/06/22 10:00
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0339(199704)16:4<331::AID-DC5>3.0.CO;2-G [pii]
AID - 10.1002/(sici)1097-0339(199704)16:4<331::aid-dc5>3.0.co;2-g [doi]
PST - ppublish
SO  - Diagn Cytopathol. 1997 Apr;16(4):331-5. doi: 
      10.1002/(sici)1097-0339(199704)16:4<331::aid-dc5>3.0.co;2-g.