PMID- 9144076
OWN - NLM
STAT- MEDLINE
DCOM- 19970724
LR  - 20141120
IS  - 1078-7852 (Print)
IS  - 1078-7852 (Linking)
VI  - 47
IP  - 4
DP  - 1995-1996
TI  - Expression of nitric oxide synthase in human peripheral blood mononuclear cells 
      and neutrophils.
PG  - 190-205
AB  - It has been clearly demonstrated in rodents that nitric oxide (NO) plays an 
      important role in host defense and immunity. However, evidence that human 
      leukocytes express inducible nitric oxide synthase (iNOS) or its products has 
      been inconclusive and a source of controversy. We report that iNOS could not be 
      detected in human monocytes, HL-60 cells, neutrophils, and T cells by Western 
      blotting analysis (< or = 10 pg) or by radiolabeled L-arginine-to-L-citrulline 
      conversion (< or = 20 pmol L-citrulline) under conditions sufficient to induce 
      iNOS in the rodent system and in human hepatocytes, which include activation with 
      cytokines, endotoxins, and/or chemoattractants. However, sensitive methods such 
      as RT-PCR and Northern blot analysis show "constitutively expressed" iNOS mRNA 
      from human monocytes, neutrophils, Jurkat cells, and HL-60 cells. This iNOS mRNA 
      is 4.4 kb and is similar to that seen in human hepatocytes and rodent 
      macrophages. In spite of the constitutive expression of mRNA in neutrophils and 
      the lack of detectable NOS activity (based on Western blotting and 
      L-arginine-to-L-citrulline conversion assay), stimulation of human neutrophils 
      unit FMLP in vitro induced the ADP-ribosylation of an intracellular NO target, 
      glyceraldehyde-3-PO4 dehydrogenase (GAPDH), in a NO-dependent manner. These 
      studies indicate that low levels of NOS protein are expressed in neutrophils (and 
      perhaps T cells and monocytes) and produce NO following stimulation. The data 
      indicate that, in addition to its phagocytic and tumoricidal activity. NO may 
      also function as an autacoid signaling molecule within the cells.
FAU - Amin, A R
AU  - Amin AR
AD  - Department of Rheumatology, Hospital for Joint Diseases Orthopaedic Institute, 
      New York, New York, USA.
FAU - Attur, M
AU  - Attur M
FAU - Vyas, P
AU  - Vyas P
FAU - Leszczynska-Piziak, J
AU  - Leszczynska-Piziak J
FAU - Levartovsky, D
AU  - Levartovsky D
FAU - Rediske, J
AU  - Rediske J
FAU - Clancy, R M
AU  - Clancy RM
FAU - Vora, K A
AU  - Vora KA
FAU - Abramson, S B
AU  - Abramson SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Inflamm
JT  - Journal of inflammation
JID - 9511967
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Adenosine Diphosphate Ribose/blood
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Separation/methods
MH  - DNA Primers/genetics
MH  - DNA, Complementary/blood/genetics
MH  - Gene Expression Regulation, Enzymologic
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/blood
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/enzymology
MH  - Leukocytes, Mononuclear/*enzymology/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neutrophils/*enzymology/metabolism
MH  - Nitric Oxide/blood
MH  - Nitric Oxide Synthase/*blood/genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/blood/genetics
MH  - Sequence Homology, Nucleic Acid
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - J Inflamm. 1995-1996;47(4):190-205.