PMID- 9144227
OWN - NLM
STAT- MEDLINE
DCOM- 19970605
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 10
DP  - 1997 May 13
TI  - Molecular cloning, sequence, expression, and processing of the interleukin 16 
      precursor.
PG  - 5273-7
AB  - Interleukin 16 (IL-16) has been shown to function as chemoattractant factor, as a 
      modulator of T-cell activation, and as an inhibitor of immunodeficiency virus 
      replication. The recent identification of inconsistencies in published IL-16 cDNA 
      nucleotide sequences led to the proposal that IL-16 is synthesized in the form of 
      a large precursor protein (pro-IL-16). To identify the true transcriptional start 
      of the IL-16 mRNA rapid amplification of cDNA ends methods were applied. The 
      complete pro-IL-16 cDNA was subsequently molecularly cloned, sequenced, and 
      expressed in COS-7 cells. We report here that pro-IL-16 is most likely 
      synthesized as a 67-kDa protein and is encoded from a major 2.6-kb transcript. 
      Recombinant pro-IL-16 polypeptides are specifically cleaved in lysates of CD8(+) 
      cells, suggesting that the naturally secreted bioactive form of IL-16 is smaller 
      than the originally published 130 amino acids fragment. Moreover, in contrast to 
      other interleukins such as IL-15, IL-16 mRNA expression is almost exclusively 
      limited to lymphatic tissues underlining the potential of IL-16 as an immune 
      regulatory molecule.
FAU - Baier, M
AU  - Baier M
AD  - Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany. 
      M.Baier@em.uni-frankfurt.de
FAU - Bannert, N
AU  - Bannert N
FAU - Werner, A
AU  - Werner A
FAU - Lang, K
AU  - Lang K
FAU - Kurth, R
AU  - Kurth R
LA  - eng
SI  - GENBANK/U82972
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-16)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (interleukin 16 precursor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Humans
MH  - Interleukin-16/*biosynthesis/chemistry/metabolism
MH  - Lymphocytes/*immunology
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Protein Precursors/*biosynthesis/chemistry/metabolism
MH  - *Protein Processing, Post-Translational
MH  - RNA, Messenger/biosynthesis
MH  - Recombinant Proteins/biosynthesis/chemistry/metabolism
MH  - *Transcription, Genetic
PMC - PMC24668
EDAT- 1997/05/13 00:00
MHDA- 1997/05/13 00:01
PMCR- 1997/11/13
CRDT- 1997/05/13 00:00
PHST- 1997/05/13 00:00 [pubmed]
PHST- 1997/05/13 00:01 [medline]
PHST- 1997/05/13 00:00 [entrez]
PHST- 1997/11/13 00:00 [pmc-release]
AID - 0723 [pii]
AID - 10.1073/pnas.94.10.5273 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 May 13;94(10):5273-7. doi: 
      10.1073/pnas.94.10.5273.