PMID- 9144384
OWN - NLM
STAT- MEDLINE
DCOM- 19970605
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 233
IP  - 1
DP  - 1997 Apr 7
TI  - Regulation of endothelial CD44 expression and endothelium-tumour cell 
      interactions by hepatocyte growth factor/scatter factor.
PG  - 1-5
AB  - Cancer metastasis involves the passage of tumour cells into and out of blood or 
      lymphatic circulatory systems and requires their interaction with the endothelial 
      cells lining these vessels. Hepatocyte growth factor/scatter factor (HGF/SF) is a 
      multifunctional protein that enhances tumour cell motility and extracellular 
      matrix invasion and has been implicated as a mediator of metastasis. In this 
      study, we have investigated the effect of HGF/SF on tumour cell-endothelial cell 
      interactions. A fluorescent tumour cell-endothelial cell attachment assay 
      demonstrated that, following endothelial monolayer stimulation with HGF/SF, 
      tumour cell attachment to endothelium is increased. Addition of anti-CD44 
      antibodies in this assay inhibited the effects of HGF/SF. Western blotting 
      studies showed that HGF/SF increased expression of the adhesion molecule CD44 in 
      endothelial cells. These results were confirmed by both immunohistochemical 
      staining and a cell-surface adhesion molecule ELISA. These results suggest that 
      HGF/SF plays a key role in the initial adhesion mechanism between tumour cells 
      and endothelial cells via up-regulation of CD44.
FAU - Hiscox, S
AU  - Hiscox S
AD  - Metastasis Research Group, University Department of Surgery, University of Wales 
      College of Medicine, Heath Park, Cardiff, United Kingdom.
FAU - Jiang, W G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hyaluronan Receptors)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Blotting, Western
MH  - Cell Adhesion
MH  - Cell Line
MH  - Endothelium, Vascular/*pathology/physiopathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Hyaluronan Receptors/*physiology
MH  - Immunohistochemistry
MH  - Neoplasm Metastasis
MH  - Neoplasms/*pathology/physiopathology
MH  - Tumor Cells, Cultured
EDAT- 1997/04/07 00:00
MHDA- 1997/04/07 00:01
CRDT- 1997/04/07 00:00
PHST- 1997/04/07 00:00 [pubmed]
PHST- 1997/04/07 00:01 [medline]
PHST- 1997/04/07 00:00 [entrez]
AID - S0006-291X(97)96388-9 [pii]
AID - 10.1006/bbrc.1997.6388 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 Apr 7;233(1):1-5. doi: 10.1006/bbrc.1997.6388.