PMID- 9144591
OWN - NLM
STAT- MEDLINE
DCOM- 19970721
LR  - 20220223
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 93
IP  - 5
DP  - 1997 May
TI  - Monocyte chemoattractant protein-1 expression and macrophage infiltration in 
      gliomas.
PG  - 518-27
AB  - While the number of reports on macrophage infiltration of gliomas is increasing, 
      the extent and mechanisms of macrophage recruitment remain unclear. To 
      investigate whether monocyte chemoattractant protein-1 (MCP-1) plays a role in 
      this process, in situ hybridisation (ISH) was performed for 22 glioblastomas 
      (GBM), 1 anaplastic astrocytoma (AA) and 4 grade II fibrillary astrocytomas (AII) 
      and reverse transcription-polymerase chain reaction was performed in 13 GBM, 1 AA 
      and 3 AII. High levels of MCP-1 mRNA were detectable in most GBM, while a lower 
      level was detected in AII. Many tumour-associated macrophages (TAM) could be 
      demonstrated by immunohistochemistry (IHC) in most GBM, while the AII contained a 
      lower number of TAM. The positive correlation between the MCP-1 level and 
      abundance of TAM suggested that MCP-1 has a role in TAM recruitment. By combining 
      ISH and IHC, high levels of MCP-1 mRNA were shown both in tumour cells and TAM. 
      Along tumour borders, reactive astrocytes and microglia also expressed MCP-1. In 
      areas with T lymphocyte infiltration, larger numbers of MCP-1-positive cells with 
      an enhanced level of expression could be identified. We propose that the 
      mechanism of macrophage recruitment is, at least partly, effected by constitutive 
      expression and T cell-mediated up-regulation of MCP-1 in tumour cells and TAM. 
      The production of MCP-1 by TAM establishes a positive amplification circuit for 
      macrophage recruitment in gliomas.
FAU - Leung, S Y
AU  - Leung SY
AD  - Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
FAU - Wong, M P
AU  - Wong MP
FAU - Chung, L P
AU  - Chung LP
FAU - Chan, A S
AU  - Chan AS
FAU - Yuen, S T
AU  - Yuen ST
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Astrocytoma/metabolism/pathology
MH  - Brain Neoplasms/*metabolism/*pathology
MH  - Chemokine CCL2/*biosynthesis
MH  - Glioblastoma/metabolism/pathology
MH  - Glioma/*metabolism/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macrophages/*physiology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/biosynthesis
MH  - T-Lymphocytes/physiology
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1007/s004010050647 [doi]
PST - ppublish
SO  - Acta Neuropathol. 1997 May;93(5):518-27. doi: 10.1007/s004010050647.