PMID- 9145036
OWN - NLM
STAT- MEDLINE
DCOM- 19970529
LR  - 20190717
IS  - 0002-9629 (Print)
IS  - 0002-9629 (Linking)
VI  - 313
IP  - 5
DP  - 1997 May
TI  - Clinical relevance of anti-HLA antibodies pre and post transplant.
PG  - 275-8
AB  - Pretransplant histocompatibility testing seeks to select compatible 
      donor-recipient pairs for transplantation. Sera from prospective renal transplant 
      recipients are screened for the presence of human leukocyte antigen (HLA) 
      antibodies to determine humoral alloimmunization. Present techniques screen 
      patient sera using a complement-dependent cytotoxicity assay and express the 
      results as percent of panel reactive antibody (PRA). However, the standard assay 
      suffers because it needs viable target cells, a variable sensitivity of cells for 
      complement, subjective evaluation, a lack of standardized methodology, and a 
      variable correlation with clinical outcomes. Alternatively, an enzyme-linked 
      immunosorbent assay (ELISA) methodology can detect IgG anti-HLA reactivity based 
      on the binding of immunoglobulin to soluble HLA class I antigens. This method 
      provides increased objectivity and reproducibility, does not require use of 
      viable target cells, and most importantly, detects immunoglobulin that is 
      reactive to HLA class I antigens. Data discussed herein suggest that identifying 
      reactive recipient sera using the enzyme-linked immunosorbent assay (ELISA) 
      (PRA-STAT, Sang Stat Med, Menlo Park, CA) methodology may be more informative 
      clinically than current standard percent of panel reactive antibody (PRA) assays.
FAU - Kerman, R H
AU  - Kerman RH
AD  - Histocompatibility and Immune Evaluation Laboratories, University of Texas 
      Medical School, Houston 77030, USA.
FAU - Orosz, C G
AU  - Orosz CG
FAU - Lorber, M I
AU  - Lorber MI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Med Sci
JT  - The American journal of the medical sciences
JID - 0370506
RN  - 0 (HLA Antigens)
RN  - 0 (Immune Sera)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Cytotoxicity Tests, Immunologic
MH  - *Enzyme-Linked Immunosorbent Assay/methods
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Immunoglobulin G/immunology
MH  - Isoantibodies/*immunology
MH  - Major Histocompatibility Complex/immunology
MH  - Transplantation Immunology/*immunology
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0002-9629(15)40116-8 [pii]
AID - 10.1097/00000441-199705000-00005 [doi]
PST - ppublish
SO  - Am J Med Sci. 1997 May;313(5):275-8. doi: 10.1097/00000441-199705000-00005.