PMID- 9145440
OWN - NLM
STAT- MEDLINE
DCOM- 19970716
LR  - 20161214
IS  - 0085-5928 (Print)
IS  - 0085-5928 (Linking)
VI  - 222
DP  - 1997
TI  - 16S ribosomal RNA-targeted oligonucleotide probes for monitoring of intestinal 
      tract bacteria.
PG  - 17-9
LID - 10.1080/00365521.1997.11720711 [doi]
AB  - BACKGROUND: The composition of a sample of faecal bacteria can be determined by 
      culturing different dilutions on specific media. However, not all bacteria can be 
      cultured and media are not always specific. With a culture-independent approach a 
      more accurate picture of the composition of the intestinal flora may be obtained. 
      METHODS: Fluorescently labelled oligonucleotide probes targeted at 16S ribosomal 
      RNA sequences specific for a bacterial genus were designed and applied for 
      fluorescence in situ hybridization (FISH) of bacteria in human faecal samples. 
      RESULTS: The mean number of Bifidobacterium spp. and the total number of 
      anaerobic bacteria per gram of faeces were determined by culturing and with the 
      probe technique. Although in both cases the number of Bifidobacterium spp. was 
      about the same, 2.38 x 10(9) and 2.45 x 10(9), it was found that the contribution 
      of Bifidobacterium spp. to the total composition is overestimated due to the 
      lower number of total anaerobic bacteria estimated by culturing. CONCLUSION: 
      Genus-specific or group-specific fluorescent 16S rRNA probes may become an 
      invaluable tool in gut ecology studies.
FAU - Welling, G W
AU  - Welling GW
AD  - Laboratorium voor Medische Microbiologie, Rijksuniversiteit Groningen, The 
      Netherlands.
FAU - Elfferich, P
AU  - Elfferich P
FAU - Raangs, G C
AU  - Raangs GC
FAU - Wildeboer-Veloo, A C
AU  - Wildeboer-Veloo AC
FAU - Jansen, G J
AU  - Jansen GJ
FAU - Degener, J E
AU  - Degener JE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Gastroenterol Suppl
JT  - Scandinavian journal of gastroenterology. Supplement
JID - 0437034
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria, Anaerobic/growth & development/*isolation & purification
MH  - Bifidobacterium/growth & development/*isolation & purification
MH  - Colony Count, Microbial
MH  - Feces/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Intestines/microbiology
MH  - *Oligonucleotide Probes
MH  - *RNA, Ribosomal, 16S
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1080/00365521.1997.11720711 [doi]
PST - ppublish
SO  - Scand J Gastroenterol Suppl. 1997;222:17-9. doi: 10.1080/00365521.1997.11720711.