PMID- 9146912
OWN - NLM
STAT- MEDLINE
DCOM- 19970625
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 2
DP  - 1997 Apr
TI  - Molecular characterization and cytological mapping of a non-repetitive DNA 
      sequence region from the W chromosome of chicken and its use as a universal probe 
      for sexing carinatae birds.
PG  - 93-101
AB  - A non-repetitive genomic DNA region of about 25 kb was cloned from the W 
      chromosome of chicken using a genomic library prepared from a single W chromosome 
      of the chicken. This region was mapped by fluorescence in situ hybridization 
      (FISH) with mitotic and lampbrush chromosomes to a position between the major 
      EcoRI family and the pericentromeric Xhol family on the W chromosome. A 0.6-kb 
      EcoRI fragment (EE0.6) subcloned from this region consists of a sequence that can 
      be obtained by the exon-trapping procedure and flanking sequences. Sequences, 
      which are closely similar to that of EE0.6, are widely conserved on the W 
      chromosomes of Carinatae birds, as revealed by Southern blot hybridization to 
      HindIII-digested female and male genomic DNAs from 18 species of birds belonging 
      to eight different taxonomic orders. The female sex of those birds can be 
      determined by the presence of an unambiguous female-specific band. For many 
      species of birds, the female sex can also be determined by polymerase chain 
      reaction (PCR) using a set of primers from the flanking sequences in the chicken 
      EE0.6.
FAU - Ogawa, A
AU  - Ogawa A
AD  - Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku 
      University, Sendai, Japan. a21668@cctu.cc.tohoku.ac.jp
FAU - Solovei, I
AU  - Solovei I
FAU - Hutchison, N
AU  - Hutchison N
FAU - Saitoh, Y
AU  - Saitoh Y
FAU - Ikeda, J E
AU  - Ikeda JE
FAU - Macgregor, H
AU  - Macgregor H
FAU - Mizuno, S
AU  - Mizuno S
LA  - eng
SI  - GENBANK/D85614
SI  - GENBANK/D85615
SI  - GENBANK/D85616
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
SB  - IM
EIN - Chromosome Res 1997 Aug;5(5):353
MH  - Animals
MH  - Base Sequence
MH  - Birds/*genetics
MH  - Blotting, Southern
MH  - Chickens/*genetics
MH  - Chromosomes/*genetics
MH  - Cloning, Molecular
MH  - DNA Probes
MH  - Female
MH  - Gene Library
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - Restriction Mapping
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Sex Determination Analysis/*methods
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1023/a:1018461906913 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Apr;5(2):93-101. doi: 10.1023/a:1018461906913.