PMID- 9146913
OWN - NLM
STAT- MEDLINE
DCOM- 19970625
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 2
DP  - 1997 Apr
TI  - Isolation of chromosome-specific paints from high-resolution flow karyotypes of 
      the sheep (Ovis aries).
PG  - 102-8
AB  - High-resolution bivariate flow karyotypes were obtained using fibroblast cell 
      lines from a sheep with a normal karyotype (2n = 54), from sheep carrying 
      Robertsonian translocation chromosomes and from sheep-hamster somatic cell 
      hybrids. By taking advantage of the presence of chromosome polymorphisms, 
      translocation chromosomes and sheep-hamster somatic cell hybrids, all sheep 
      chromosomes were isolated by flow sorting. Chromosome-specific paints were 
      generated from each sorted peak using degenerate oligonucleotide-primed 
      polymerase chain reaction (DOP-PCR). The sheep chromosome present in each peak 
      was identified by chromosome-specific microsatellite analysis of the DOP-PCR 
      products and fluorescence in situ hybridization (FISH) onto DAPI-banded sheep 
      metaphase chromosomes. The chromosome-specific DNA obtained in this study can be 
      used for the production of genomic libraries and as a resource for mapping 
      randomly cloned DNA sequences that will greatly aid the construction of genetic 
      and physical maps in the sheep. The chromosome-specific paints will facilitate 
      chromosome identification and contribute to the study of karyotype evolution in 
      the sheep and related species.
FAU - Burkin, D J
AU  - Burkin DJ
AD  - Pathology Department, University of Cambridge, UK. maf12@mole.bio.cam.ac.uk
FAU - O'Brien, P C
AU  - O'Brien PC
FAU - Broad, T E
AU  - Broad TE
FAU - Hill, D F
AU  - Hill DF
FAU - Jones, C A
AU  - Jones CA
FAU - Wienberg, J
AU  - Wienberg J
FAU - Ferguson-Smith, M A
AU  - Ferguson-Smith MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Separation
MH  - Cricetinae
MH  - DNA Probes
MH  - Fibroblasts
MH  - Flow Cytometry
MH  - Fluorescent Dyes
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles
MH  - *Karyotyping
MH  - Male
MH  - Microsatellite Repeats/genetics
MH  - Polymerase Chain Reaction/methods
MH  - Sheep/*genetics
MH  - Translocation, Genetic
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1023/a:1018414123751 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Apr;5(2):102-8. doi: 10.1023/a:1018414123751.