PMID- 9152427
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 78 ( Pt 5)
DP  - 1997 May
TI  - Presence of a novel hamster oral papillomavirus in dysplastic lesions of hamster 
      lingual mucosa induced by application of dimethylbenzanthracene and excisional 
      wounding: molecular cloning and complete nucleotide sequence.
PG  - 1087-93
AB  - A combination of 9,10-dimethyl-1,2-benzanthracene (DMBA) application and 
      excisional wounding on the lingual tips of Syrian Golden hamsters (Mesocricetus 
      auratus) induces dysplastic and malignant mucosal lesions. Papillomavirus 
      genus-specific antigen and viral particles, measuring 55 nm in diameter, were 
      demonstrated in the nuclei of squamous cells of dysplastic lesions showing 
      koilocytotic change. In this study, we cloned a circular genome at a single Kpnl 
      site from one of these dysplastic lesions. The genomic sequence of this clone, 
      consisting of 7647 bp, was shown to be that of a novel papillomavirus with a 
      conserved genomic organization. We named the new virus hamster oral 
      papillomavirus (HOPV). All dysplastic lesions induced by this combination of DMBA 
      application and excisional wounding contained viral DNA. Although Southern blot 
      hybridization analysis could not detect the HOPV genome, PCR analysis 
      demonstrated the latent HOPV genome in the tongue and skin of an untreated 
      hamster. These results suggest that latently present HOPV genome is reactivated 
      by the DMBA/wounding procedures. Lingual HOPV infection may be an important model 
      for gaining insight into the interactions between papillomavirus infection, 
      chemical carcinogens and physical irritations in carcinogenesis or malignant 
      transformation.
FAU - Iwasaki, T
AU  - Iwasaki T
AD  - Department of Pathology, National Institute of Health, Tokyo, Japan. 
      tiwasaki@nih.go.jp
FAU - Maeda, H
AU  - Maeda H
FAU - Kameyama, Y
AU  - Kameyama Y
FAU - Moriyama, M
AU  - Moriyama M
FAU - Kanai, S
AU  - Kanai S
FAU - Kurata, T
AU  - Kurata T
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Carcinogens)
RN  - 0 (DNA, Viral)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene/*pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Carcinogens/*pharmacology
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Viral
MH  - Genome, Viral
MH  - Mesocricetus
MH  - Molecular Sequence Data
MH  - Mouth Mucosa/pathology/virology
MH  - Papillomaviridae/*genetics
MH  - Papillomavirus Infections/etiology/pathology/*virology
MH  - Sequence Analysis, DNA
MH  - Tumor Virus Infections/etiology/pathology/*virology
MH  - *Wounds and Injuries
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1099/0022-1317-78-5-1087 [doi]
PST - ppublish
SO  - J Gen Virol. 1997 May;78 ( Pt 5):1087-93. doi: 10.1099/0022-1317-78-5-1087.