PMID- 9153541
OWN - NLM
STAT- MEDLINE
DCOM- 19970529
LR  - 20190718
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 40
IP  - 5
DP  - 1997 May
TI  - In situ expression of B7 and CD28 receptor families in skin lesions of patients 
      with lupus erythematosus.
PG  - 814-21
AB  - OBJECTIVE: To examine the expression of costimulatory molecules of the B7 and 
      CD28 receptor families in active skin lesions of patients with systemic lupus 
      erythematosus (SLE), subacute cutaneous lupus erythematosus (SCLE), and chronic 
      discoid lupus erythematosus (CDLE). METHODS: The in situ expression of B7-1, 
      B7-2, BB-1, and CD28 was studied by immunohistochemistry, and B7-1 and B7-2 RNA 
      expression was examined by reverse transcription-polymerase chain reaction. 
      RESULTS: Only in lesional skin from SLE, SCLE, and CDLE patients did dermal and 
      epidermal antigen-presenting cells (APC) express B7-1 and B7-2, particularly when 
      in apposition to CD28+ T cells. These B7-1+ and B7-2+ APC bound CTLA-4 fusion 
      protein. In lesional (but not in nonlesional) skin, keratinocytes expressed BB-1. 
      The majority of infiltrating T cells were CD28+. B7-1 and B7-2 RNA were expressed 
      in lesional skin from SLE, SCLE, and CDLE patients; when dermis was separated 
      from epidermis, only faint B7-1 and B7-2 RNA signals were detectable in the 
      epidermis, indicating that dermal but not epidermal cells were the major source 
      of B7-1 and B7-2 RNA. During treatment, both B7-1 and B7-2 protein and RNA 
      expression were reduced. CONCLUSION: These in situ findings suggest that 
      costimulation via the B7-CD28 pathway may be important for the generation and/or 
      propagation of T cell activity in skin lesions of humans with lupus 
      erythematosus. Thus, the manipulation of this pathway (e.g., by CTLA-4 fusion 
      protein) could be an important target for the development of future therapies for 
      LE.
FAU - Denfeld, R W
AU  - Denfeld RW
AD  - University of Freiburg, Germany.
FAU - Kind, P
AU  - Kind P
FAU - Sontheimer, R D
AU  - Sontheimer RD
FAU - Schopf, E
AU  - Schopf E
FAU - Simon, J C
AU  - Simon JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD28 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigens, CD/*biosynthesis/genetics
MH  - B7-1 Antigen/*biosynthesis/genetics
MH  - B7-2 Antigen
MH  - CD28 Antigens/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - Lupus Erythematosus, Cutaneous/*metabolism
MH  - Lupus Erythematosus, Discoid/metabolism
MH  - Lupus Erythematosus, Systemic/metabolism
MH  - Membrane Glycoproteins/*biosynthesis/genetics
MH  - RNA, Messenger/metabolism
MH  - Skin/metabolism
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1002/art.1780400507 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1997 May;40(5):814-21. doi: 10.1002/art.1780400507.