PMID- 9154391
OWN - NLM
STAT- MEDLINE
DCOM- 19970819
LR  - 20190831
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 16
IP  - 5
DP  - 1997 May
TI  - The lacrimal gland expresses nuclear retinoid X receptors.
PG  - 511-5
AB  - PURPOSE: The lacrimal gland expresses nuclear retinoic acid receptors. This 
      suggests that retinoids are involved in control of gene expression in the 
      lacrimal gland. Retinoid X receptors (RXRs) form heterodimers with and are 
      required for activation of retinoic acid receptors. The purpose of this study was 
      to identify retinoid X receptors in the lacrimal gland. METHODS: Total RNA was 
      purified from rat, rabbit and human lacrimal glands and from cultured rat 
      lacrimal cells. RNA was analyzed by northern blotting using cDNA probes for RXR 
      alpha, RXR beta and RXR gamma. Nuclear protein extracts from rat and rabbit 
      lacrimal glands were probed for RXR beta by immunoblotting, using a mouse 
      monoclonal antibody. RESULTS: RXR alpha mRNA transcripts (5 kb) and RXR beta mRNA 
      transcripts (3.3 kb) are present in the lacrimal glands of all species studied 
      and in cultured rat lacrimal cells. RXR gamma mRNA (1.9 kb) was detected only in 
      the rabbit lacrimal gland. RXR beta is expressed as a 50 kDa protein in rat and 
      rabbit lacrimal glands. CONCLUSIONS: This study confirms the presence of RXRs in 
      the lacrimal gland, thereby supporting a role for retinoids and their nuclear 
      receptors in the control of gene transcription in the lacrimal gland.
FAU - Ubels, J L
AU  - Ubels JL
AD  - Biology Department, Calvin College, Grand Rapids, MI 49546, USA.
FAU - Sulahian, T H
AU  - Sulahian TH
FAU - Viel, M D
AU  - Viel MD
LA  - eng
GR  - R01 EY04650/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Humans
MH  - Isomerism
MH  - Lacrimal Apparatus/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nuclear Proteins/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Retinoic Acid/genetics/*metabolism
MH  - Retinoid X Receptors
MH  - Transcription Factors/genetics/*metabolism
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1076/ceyr.16.5.511.7045 [doi]
PST - ppublish
SO  - Curr Eye Res. 1997 May;16(5):511-5. doi: 10.1076/ceyr.16.5.511.7045.