PMID- 9155585
OWN - NLM
STAT- MEDLINE
DCOM- 19970602
LR  - 20190501
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 40
IP  - 1
DP  - 1997 Jan
TI  - Antineutrophil cytoplasm autoantibodies against 
      bactericidal/permeability-increasing protein in inflammatory bowel disease.
PG  - 105-9
AB  - BACKGROUND: Bactericidal/permeability-increasing protein (BPI), a constituent of 
      primary neutrophil granules, is a potent natural antibiotic and an antineutrophil 
      cytoplasm antibody (ANCA) antigen in cases of vasculitis in which the target 
      antigen is neither myeloperoxidase (MPO) nor proteinase-3 (PR3). AIM: To 
      investigate BPI as a possible target antigen for ANCAs in inflammatory bowel 
      disease. METHODS: ANCAs were detected by routine immunofluorescence (IIF) and 
      solid phase enzyme linked immunosorbent assay (ELISA) performed for antibodies to 
      the purified neutrophil granule proteins; MPO, PR3, cathepsin-G, lactoferrin, and 
      BPI in serum samples from 88 patients with inflammatory bowel disease (36 with 
      Crohn's disease, 52 with ulcerative colitis). Thirty patients with bacterial 
      enteritis acted as controls. RESULTS: Significantly more patients with ulcerative 
      colitis were ANCA positive by IIF (60%) than patients with Crohn's disease (28%) 
      or infectious enteritis (23%) (p < 0.001). IgG anti-BPI antibodies were present 
      in 29% of patients with ulcerative colitis, 14% of patients with Crohn's disease, 
      and 23% of patients with infectious enteritis, occurring in 44% of those patients 
      with inflammatory bowel disease who were ANCA positive by IIF. Antibodies to 
      other ANCA antigens were rare. The presence of ANCAs was not related to either 
      disease activity or extent; presence of anti-BPI antibodies was significantly 
      related to both a lower serum albumin concentration (p = 0.001) and a higher 
      erythrocyte sedimentation rate (p = 0.02) in patients with ulcerative colitis, 
      and to colonic involvement in patients with Crohn's disease (p = 0.01). 
      CONCLUSION: BPI is a significant minority target antigen for ANCAs in 
      inflammatory bowel disease that seems related to colonic Crohn's disease and 
      disease activity in ulcerative colitis. Anti-BPI antibodies occur in infectious 
      enteritis.
FAU - Walmsley, R S
AU  - Walmsley RS
AD  - Department of Medicine, Royal Free Hospital School of Medicine, London.
FAU - Zhao, M H
AU  - Zhao MH
FAU - Hamilton, M I
AU  - Hamilton MI
FAU - Brownlee, A
AU  - Brownlee A
FAU - Chapman, P
AU  - Chapman P
FAU - Pounder, R E
AU  - Pounder RE
FAU - Wakefield, A J
AU  - Wakefield AJ
FAU - Lockwood, C M
AU  - Lockwood CM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Blood Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Membrane Proteins)
RN  - 0 (bactericidal permeability increasing protein)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.21.- (Lactoferrin)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.20 (CTSG protein, human)
RN  - EC 3.4.21.20 (Cathepsin G)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Antineutrophil Cytoplasmic/*analysis
MH  - Antimicrobial Cationic Peptides
MH  - Blood Proteins/*immunology
MH  - Case-Control Studies
MH  - Cathepsin G
MH  - Cathepsins/immunology
MH  - Colitis, Ulcerative/*immunology
MH  - Crohn Disease/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Immunoglobulin G/*analysis
MH  - Lactoferrin/immunology
MH  - Male
MH  - *Membrane Proteins
MH  - Middle Aged
MH  - Peroxidase/immunology
MH  - Prospective Studies
MH  - Serine Endopeptidases/*immunology
PMC - PMC1027017
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
PMCR- 1997/01/01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PHST- 1997/01/01 00:00 [pmc-release]
AID - 10.1136/gut.40.1.105 [doi]
PST - ppublish
SO  - Gut. 1997 Jan;40(1):105-9. doi: 10.1136/gut.40.1.105.